Recombinant Mouse Sarcoplasmic/endoplasmic reticulum calcium ATPase 1 (Atp2a1), partial

Code CSB-YP819187MO
MSDS
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Source Yeast
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Code CSB-EP819187MO
MSDS
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Source E.coli
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Code CSB-EP819187MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819187MO
MSDS
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Source Baculovirus
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Code CSB-MP819187MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Atp2a1
Uniprot No.
Alternative Names
Atp2a1; Sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1; SR Ca(2+)-ATPase 1; EC 7.2.2.10; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type; fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Gene References into Functions
  1. Our results indicate that the phospholipid headgroup PC:PE negatively correlated and could potentially contribute to reductions in SERCA activity seen in functionally overloaded plantaris. PMID: 29534725
  2. CAPN3 deficiency leads to degradation of SERCA proteins and Ca2+ dysregulation in the skeletal muscle. PMID: 27055500
  3. Ebf3 binds directly to the promoter of Atp2a1 and synergises with MyoD in the induction of Atp2a1 PMID: 24786561
  4. Formalin evokes calcium transients from the endoplasmatic reticulum via SERCA1-dependent, TRPA1-independent mechanism that may underlie formaldehyde-induced pan-neuronal excitation and subsequent inflammation. PMID: 25875358
  5. SERCA1b is considered to play an essential role in the regulation of [Ca2+]i and its ab ovo gene silencing results in decreased skeletal muscle differentiation. PMID: 25893964
  6. Data show that in healthy transgenic mice, cardiac-specific sarcoplasmic reticulum calcium ATPase SERCA1a expression increased active cell-surface glucose transporter GLUT4 and glucose uptake in the myocardium. PMID: 25615793
  7. Data suggest that the cardiac depressant properties of neuronostatin possibly associated with loss of sarcoplasmic reticulum Ca2+-ATPase SERCA phosphorylation. PMID: 25012062
  8. Results indicated that sine oculis homeobox 1 (Six1) overexpression could significantly promote the expression of fast-type muscle genes Atp2a1, Srl, and Mylpf. PMID: 23613228
  9. Protein levels of CSQ1, SERCA1, and SERCA2 are re-adjusted in skeletal muscles depending on the demands of diverse exercise training programs. PMID: 22466636
  10. dystrophic phenotype observed in delta-sarcoglycan-null (Sgcd(-/-)) mice and dystrophin mutant mdx mice is dramatically improved by skeletal muscle-specific overexpression of sarcoplasmic reticulum Ca(2+) ATPase 1 (SERCA1) PMID: 21285509
  11. Targeted disruption of the gene encoding this protein in the sarco(endo)plasmic reticulum impairs diaphragm function and is lethal in neonatal mice. PMID: 12556521
  12. Sarcolipin is a novel regulator of SERCA pump activity, and its inhibitory effect can be reversed by beta-adrenergic agonists. PMID: 16365042
  13. The results indicate that SERCA overexpression can reduce the *OH-induced contractile dysfunction in murine myocardium, whereas a reduced SR Ca(2+)-ATPase activity aggravates this injury. PMID: 16798816
  14. Our studies point to an important regulation of SERCA1b expression at the protein level and hints to a role in the growth of the developing muscle. PMID: 17010426
  15. Increased TR expression in the hypertrophied heart is associated with an improvement in contractile function and increased SERCA expression. PMID: 17317766
  16. In dystrophin-deficient mdx mice the serca1 is overpression in laryngeal muscles. PMID: 19301368

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Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein. Sarcoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIA subfamily
Database Links
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