Recombinant Mouse Sentrin-specific protease 2 (Senp2)

Code CSB-YP856342MO
MSDS
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Source Yeast
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Code CSB-EP856342MO
MSDS
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Source E.coli
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Code CSB-EP856342MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856342MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP856342MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Senp2; Smt3ip2; Supr1; Sentrin-specific protease 2; EC 3.4.22.-; Axam2; SUMO-1 protease 1; SuPr-1; SUMO-1/Smt3-specific isopeptidase 2; Smt3ip2; Sentrin/SUMO-specific protease SENP2
Species
Mus musculus (Mouse)
Expression Region
1-588
Target Protein Sequence
MYRWLAKVLG TILRLCERPA PGARALLKRR RSSSTLFSTA VDTDEIPAKR PRLDCFIHQV KNSLYNAASL FGFPFQLTTK PMVSSACNGT RNVAPSGEVF SNSSSCELMS SGSCSSMLKL GNKSPNGISD YPKIRVTVTR DQPRRVLPSF GFTLKSEGYN RRPSGRRHSK SNPESSLTWK PQEQGVTEMI SEEGGKGVRR PHCTVEEGVQ KDEREKYRKL LERLKEGAHG STFPPTVSHH SSQRIQMDTL KTKGWVEEQN HGVRTTHFVP KQYRVVETRG PLCSMRSEKR YSKGKADTEK VVGLRFEKEG TRGHQMEPDL SEEVSARLRL GSGSNGLLRR KISVLEIKEK NFPSKEKDRR TEDLFEFTED MEKEISNALG HGPPDEILSS AFKLRITRGD IQTLKNYHWL NDEVINFYMN LLVERSKKQG YPALHAFSTF FYPKLKSGGY QAVKRWTKGV NLFEQELVLV PIHRKVHWSL VVMDLRKKCL KYLDSMGQKG HRICEILLQY LQDESKTKRN TDLNLLEWTH YSMKPHEIPQ QLNGSDCGMF TCKYADYISR DKPITFTQHQ MPLFRKKMVW EILHQQLL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Protease that catalyzes two essential functions in the SUMO pathway. The first is the hydrolysis of an alpha-linked peptide bond at the C-terminal end of the small ubiquitin-like modifier (SUMO) propeptides, SUMO1, SUMO2 and SUMO3 leading to the mature form of the proteins. The second is the deconjugation of SUMO1, SUMO2 and SUMO3 from targeted proteins, by cleaving an epsilon-linked peptide bond between the C-terminal glycine of the mature SUMO and the lysine epsilon-amino group of the target protein. May down-regulate CTNNB1 levels and thereby modulate the Wnt pathway. Deconjugates SUMO2 from MTA1. Plays a dynamic role in adipogenesis by desumoylating and promoting the stabilization of CEBPB. Isoform 3 activates transcription.
Gene References into Functions
  1. Data suggest that SENP2 in hepatic stellate cells is involved in regulation of apoptosis in these cells and in development/reversion of liver fibrosis (here, carbon tetrachloride-induced liver fibrosis). PMID: 29535048
  2. Expression of the SENP2 gene was suppressed by theobromine. In vivo knockdown studies showed that AR1 knockdown in mice attenuated the anti-adipogenic effects of theobromine in younger mice. Theobromine suppresses adipocyte differentiation and induced C/EBPbeta degradation by increasing its sumoylation. PMID: 28965824
  3. SENP2 regulates Drp1 sumoylation and stability critical for mitochondrial morphogenesis.SENP2 plays role in mitochondria mediated neural degeneration. PMID: 25299344
  4. This study identified an animal model of SUDEP, which is caused by alteration of a posttranslational protein modification pathway (SUMOylation) on a unique voltage-gated potassium channel (Kv7.2/Kv7.3) PMID: 25189211
  5. These results reveal the important role of SENP2 in the regulation of myostatin expression and myogenesis. PMID: 24344126
  6. SENP2 represses glycolysis and shifts glucose metabolic strategy PMID: 23691130
  7. Data suggest that SENP2 regulates antiviral innate immunity by deSUMOylating IRF3 and conditioning it for ubiquitination and degradation, and provide an example of cross-talk between the ubiquitin and SUMO pathways in innate immunity. PMID: 22028379
  8. a mechanism underlying the SENP2-mediated regulation of Mdm2 that is critical for genome integrity in p53-dependent stress responses. PMID: 21183956
  9. SENP2 de-SUMOylates PPARgamma in myotubes, and de-SUMOylation of PPARgamma selectively increases the expression of some PPARgamma target genes. PMID: 20950277
  10. Senp2 is essential for suppression of polycomb group protein-mediated gene silencing during embryonic development. PMID: 20417598
  11. These results establish a critical role for SENP2 in the regulation of adipogenesis by desumoylation. PMID: 20194620
  12. SUMO-1 protease-1 regulates gene transcription through PML. PMID: 12419228
  13. data reveal a key genetic pathway, SENP2-Mdm2-p53, underlying trophoblast lineage development, suggesting its pivotal role in cell cycle progression of mitosis and endoreduplication. PMID: 19090619

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Subcellular Location
[Isoform 1]: Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side.; [Isoform 2]: Cytoplasm. Cytoplasmic vesicle.; [Isoform 3]: Nucleus, PML body.
Protein Families
Peptidase C48 family
Tissue Specificity
Highly expressed in testis. Detected in brain, heart and thymus.
Database Links
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