Recombinant Mouse Serum paraoxonase/lactonase 3 (Pon3)

Code CSB-YP737067MO
MSDS
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Source Yeast
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Code CSB-EP737067MO
MSDS
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Source E.coli
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Code CSB-EP737067MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP737067MO
MSDS
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Source Baculovirus
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Code CSB-MP737067MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pon3
Uniprot No.
Alternative Names
Pon3; Serum paraoxonase/lactonase 3; EC 3.1.1.2; EC 3.1.1.81; EC 3.1.8.1
Species
Mus musculus (Mouse)
Expression Region
2-354
Target Protein Sequence
GKLVALTLL GACLALIGER LLNFRERVST TREIKATEPQ NCHLIEGLEN GSEDIDILPS GLAFISTGLK YPGMPAFAPD KPGRIFLMDL NEQNPEAQAL EISGGLDQES LNPHGISTFI DKDNTAYLYV VNHPNMDSTV EIFKFEEQQR SLIHLKTLKH ELLKSVNDIV VLGPEQFYAT RDHYFTSYFL VLLEMILDPH WTSVVFYSPK EVKVVAQGFS SANGITVSLD QKFVYVADVT AKNIHIMKKH DNWDLTPVKV IQLGTLVDNL TVDPATGDIL AGCHPNPMKL LIYNPEDPPG SEVLRIQDSL SDKPRVSTLY ANNGSVLQGS TVASVYHKRM LIGTIFHKAL YCDL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.
Gene References into Functions
  1. study suggests a role for PON3 in the metabolism of lipid and bile acid as well as protection against atherosclerosis, gallstone disease, and obesity PMID: 25477283
  2. PON3 is upregulated in cancer tissues and protects against mitochondrial superoxide-mediated cell death. PMID: 22441669
  3. investigation of effect of knockout of Pon3 in embryo: Data suggest that Pon3-/- mice exhibit increased early embryonic and neonatal mortality. PMID: 21952037
  4. mouse Pon3 is expressed in a wide range of tissues, and that its expression is temporally controlled PMID: 20221872
  5. PON3 proteins were present in the vast majority of tissues investigated. mRNA for these proteins was also expressed in most of these tissues suggesting local production and the ability to respond in situ to inflammatory stimuli. PMID: 20931267
  6. The anti-atherogenic biological activities were studied in vitro using serum or cell cultures, and also in vivo, using PON 1/2/3 knockout or transgenic mice, as well as humans - healthy volunteers and atherosclerotic patients. PMID: 19082953
  7. The directed evolution and characterization of recombinant variants of serum paraoxonase PON3, is described. PMID: 19492856

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Subcellular Location
Secreted, extracellular space.
Protein Families
Paraoxonase family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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