Recombinant Mouse Sperm-associated antigen 16 protein (Spag16)

Code CSB-YP815676MO
MSDS
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Source Yeast
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Code CSB-EP815676MO
MSDS
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Source E.coli
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Code CSB-EP815676MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP815676MO
MSDS
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Source Baculovirus
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Code CSB-MP815676MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spag16
Uniprot No.
Alternative Names
Spag16; Pf20; Sperm-associated antigen 16 protein; Pf20 protein homolog
Species
Mus musculus (Mouse)
Expression Region
1-639
Target Protein Sequence
MAAPSGVPPL RVLEELGIGL SPTGEVTEAV TSEGAYYLEQ VTITETSEDE CEYEEIPDDN FSIPEGEEDL EKAIHIIGEQ ARDIHILEQQ TILPARNVMQ EAIEDFLCNF LIKMGMTRTL DCFQAEWYEL IQKKGSDFKG LGNVPDVYSQ VMLLETENKN LKKELKHFKQ AAEKAKEDLL RTQKERDFHR MHHKRIVQEK NKLIADLKGL KLHYASYEPT IRVLHEKHHA LLKEKMLTSL ERDRAVGKIS GLQATLKNID MGHIQVPVIK GSYESASITR ESGDRAGHSC EKENSSEGPT QKSLREAREE VGYKSKLKNE KKDSEFPVDM QPDPNVTSCT ENVSAAKFDY KLNNIFRLHE LPVSCIVMHP CRDYLISCSE DRLWKMVGLP QGNVLLTGSG HTDWLSGCCF HPSGSKLATS SGDSTIKLWD LNKGECTLTL EGHNHAVWSC TWHSCGDFVA SASLDMTSKI WDVNSERCRY TLYGHTDSVN SIEFFPFSNI LLTASADKTL SVWDARTGKC EQSLYGHMHS VNDATFTPRG HIIASCDARG VTKLWDFRKL IPIVSIDVGP SSGNEVNFDQ SGRVLAQASA NGIIHLLDLK SGQIHKLVGH ESEVHSVVFS HLGENLYSGG SDGTIRLWI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Necessary for sperm flagellar function. Plays a role in motile ciliogenesis. May help to recruit STK36 to the cilium or apical surface of the cell to initiate subsequent steps of construction of the central pair apparatus of motile cilia.
Gene References into Functions
  1. The distinct nuclear localization of SPAG16S and its ability to modulate Spag16L mRNA expression suggest that SPAG16S plays an important role in the gene expression machinery of male germ cells. PMID: 21655194
  2. Functional deficiencies and a reduced response to calcium in the flagellum of mouse sperm lacking SPAG16L. PMID: 20042536
  3. In sperm, SPAG16L is found in the central apparatus of the axoneme. We conclude that SPAG16L is essential for sperm flagellar function. PMID: 16382026
Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, cilium axoneme. Cytoplasm, cytoskeleton, flagellum axoneme. Cell projection, cilium, flagellum.
Tissue Specificity
Expressed in testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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