Recombinant Mouse Src kinase-associated phosphoprotein 1 (Skap1)

Code CSB-YP662390MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP662390MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP662390MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP662390MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP662390MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Skap1; Scap1; Src kinase-associated phosphoprotein 1; Src family-associated phosphoprotein 1
Species
Mus musculus (Mouse)
Expression Region
1-355
Target Protein Sequence
MQAVALPEEI CWLLEDTEDF LAEGLQNENL SPGAQDQRAH ILRGFQQIKS RYCWDFQPQG GDLGQDGSDD NLSGTHGPPL TSEASFWSDY QDEGIEDILR GAQELDSVIK QGYLEKKSKD HSFFGSEWQK RWCVISRGLF LYYANEKSKQ PKGTFLIKGY SVRMAPHLRK DSKKESCFEL ISQDRRSYEF TASSPAEARD WVDQISFLLK DLSSLTIPFE EEEEEEEEEE KEEEEMYNDV DGFDSPRSGS QCRAMALPEP TEKEEDIYEV LPDDDDLEED TCGAHRRRVD YADYYQGLWD CHGDQPDELS FQRGDLIRIL SKEYNMYGWW VGELNSVIGI VPKDYLTTAF EMEGI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Positively regulates T-cell receptor signaling by enhancing the MAP kinase pathway. Required for optimal conjugation between T-cells and antigen-presenting cells by promoting the clustering of integrin ITGAL on the surface of T-cells. May be involved in high affinity immunoglobulin epsilon receptor signaling in mast cells.
Gene References into Functions
  1. SKAP1 can affect the function of talin in T-cells needed for optimal T-cell/dendritic cell conjugation PMID: 26905930
  2. this study shows that Skap1-deficient (skap1-/-) mice are highly resistant to the induction of collagen-induced arthritis PMID: 27181093
  3. In this study, the authors identified that the ADAP-SKAP55 signaling module reduced CD8(+) cytotoxic T lymphocyte cytotoxicity and enhanced PD-1 expression in a Fyn-, Ca(2+)-, and NFATc1-dependent manner. PMID: 25851535
  4. Data suggest that the presence or absence of associated SKAP55 defines functionally distinct pools of ADAP. PMID: 21525391
  5. HPK1 associates with SKAP1 to negatively regulate Rap1-mediated B-lymphocyte adhesion. PMID: 20824186
  6. SKAP1 is dispensable for both CXCL12 and CCL21 induced T-cell migration. PMID: 19883688
  7. findings define a T cell receptor "inside-out" pathway via N-SKAP1-C-RapL that regulates T cell adhesion, motility, and arrest times with dendritic cells in lymph nodes. PMID: 20346707
  8. Results identify a clear effector role for SKAP-55 in LFA-1 adhesion in peripheral T cells and demonstrate that dependency on SKAP-55 and ADAP differs among T cells and differs with the strength of the TCR signal. PMID: 17646386

Show More

Hide All

Subcellular Location
Cytoplasm. Nucleus. Cell membrane.
Protein Families
SKAP family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1