Product Details

Purity

>85% (SDS-PAGE)

Target Names

Soat2

Uniprot No.

O88908

Alternative Names

Soat2; Acact-2; Acat2; Sterol O-acyltransferase 2; EC 2.3.1.26; Acyl-coenzyme A:cholesterol acyltransferase 2; ACAT-2; Cholesterol acyltransferase 2

Species

Mus musculus (Mouse)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.

Gene References into Functions

These studies demonstrate that SOAT2 is a negative regulator of LXR-stimulated fecal neutral sterol loss in mice. PMID: 26729489
Hepatic knockdown of SOAT2 in apoB100-only, LDLr(-/-) mice resulted in remodeling of aortic atherosclerotic lesions into a stable phenotype, suggesting SOAT2 is a viable target for the treatment of atherosclerosis. PMID: 26229140
SOAT2 causes hepatic cholesterol to be swiftly mobilized and packaged onto nascent lipoproteins that feed cholesterol into the TICE pathway for fecal excretion. PMID: 24901470
effect the loss of SOAT2 function has on tissue esterified cholesterol sequestration in lysosomal acid lipase-deficient mice in a model of cholesteryl ester storage disease PMID: 25450374
Aortic atherosclerosis development was significantly lower in SOAT2 knock-out mice. PMID: 25239141
ACAT2 increases cholesterol absorption efficiency by providing cholesterol esters for chylomicron transport, but 1 copy of the Acat2 gene, providing approximately 50% of ACAT2 mRNA and enzyme activity, was as effective as 2 copies in promoting cholesterol absorption. PMID: 22045928
ACAT2-derived cholesterol esters have a crucial role in the development of atherosclerosis in mice PMID: 12538880
ACAT2 displays a greater capacity than ACAT1 to differentiate cholesterol from sitosterol PMID: 12975367
ACAT2-derived cholesteryl ester is the predominant atherogenic lipid in blood. PMID: 15486318
ACAT2 deficiency limits cholesterol absorption. Extent it impacts hepatic cholesterol homeostasis depends on cholesterol intake. Loss of ACAT2 activity may result in unesterified cholesterol being absorbed via ABCA1-mediated basolateral efflux pathway. PMID: 15486928

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily

Database Links

KEGG: mmu:223920

STRING: 10090.ENSMUSP00000023806

UniGene: Mm.358862

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Recombinant Mouse Sterol O-acyltransferase 2 (Soat2), partial

Product Details

Customer Reviews and Q&A

Target Background

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