Recombinant Mouse Synaptic vesicular amine transporter(Slc18a2),partial

Code CSB-YP803926MO
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Source Yeast
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Code CSB-EP803926MO
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Source E.coli
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Code CSB-EP803926MO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP803926MO
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Source Baculovirus
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Code CSB-MP803926MO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Slc18a2
Uniprot No. Q8BRU6
Alternative Names Slc18a2; Vmat2; Synaptic vesicular amine transporter; Monoamine transporter; Solute carrier family 18 member 2; Vesicular amine transporter 2; VAT2
Species Mus musculus (Mouse)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Involved in the ATP-dependent vesicular transport of biogenic amine neurotransmitters. Pumps cytosolic monoamines including dopamine, norepinephrine, serotonin, and histamine into synaptic vesicles. Requisite for vesicular amine storage prior to secretion via exocytosis.
Gene References into Functions
  1. results confirm the role of VMAT2 in the protection of vulnerable nigrostriatal dopamine neurons and may also provide new insight into the side effects of L-DOPA treatments in Parkinson's disease PMID: 27287315
  2. Study identified an importance for VMAT2-mediated neurotransmission in mediating hedonic feeding and binge-like eating behavior, providing novel insight on the interactive roles of leptin and dopamine, two of the most extensively studied brain systems, in obesity development. PMID: 28560316
  3. In vivo imaging of the striatal vesicular monoamine transporter type 2 (VMAT2) distribution using 18F-FP-(+)-DTBZ animal PET is a useful method to evaluate the efficacy of therapeutic drugs i.e., magnolol, for the management of Parkinson's disease . PMID: 28257461
  4. Intravenous use of a psychostimulant drug containing methcathinone (ephedrone) and manganese causes an irreversible extrapyramidal syndrome; study reproduced the syndrome in mice to evaluate dopaminergic damage. Increased [(11)C]dihydrotetrabenazine binding may indicate vesicular monoamine transporter type 2 (VMAT2) function is altered. PMID: 27693414
  5. In contrast to their loss of catecholamine neurons of the substantia nigra and locus ceruleus, aged VMAT2 LO mice do not exhibit a change in the number of serotonergic (TPH2+) neurons within the dorsal raphe PMID: 26428905
  6. Rotenone induces dopamine neuron death through a series of sequential events including microtubule destabilization, JNK3 activation, VMAT2 inhibition, accumulation of cytosolic dopamine, and generation of ROS. PMID: 25496994
  7. increased release of dopamine and neuroprotection from MPTP toxicity in the VMAT2-overexpressing mice suggest that interventions aimed at enhancing vesicular capacity may be of therapeutic benefit in Parkinson disease PMID: 24979780
  8. The coordinate loss of dopamine and norepinephrine neurons in VMAT2 LO mice parallels the pattern of neurodegeneration that occurs in human PD. PMID: 24025942
  9. findings support the idea that altered DAT and VMAT2 expression affect age-related changes in dopaminergic function. PMID: 23978383
  10. Data suggest that pancreatic islets of mice and rats do not contain VMAT2, except in mast cells and sympathetic nerve terminals that innervate the islets. PMID: 23404442
  11. VMAT2 expression was increased by Nurr1 gene activation in lactacystin-lesioned mice. PMID: 22483304
  12. Data show that alpha-synuclein transcription is inversely correlated with neurite growth and VMAT2 expression, and inhibition of RhoA leads to a decrease in alpha-synuclein. PMID: 21699982
  13. The expression of VMAT2 is increased significantly in hippocampal formation of 14-3-3 epsilon knockout mouse model compared to that of wild-type littermates. PMID: 20815037
  14. VMAT2 up-regulation might be a compensatory mechanism to restore and maintain synaptic transmission in dopaminergic midbrain neurons during nicotine withdrawal. PMID: 19835933
  15. mice with reduced expression of VMAT2 (heterozygote) are more sensitive to the neurotoxic effects of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine and methamphetamine PMID: 12428733
  16. The vesicular monoamine content regulates VMAT2 activity through Galphaq in mouse platelets. PMID: 12604601
  17. Deletions of the VMAT2 gene increase ethanol consumption in male knockout mice. PMID: 12655306
  18. The relative importance of the DAT and terminal autoreceptors as control mechanisms in the actions of amphetamine are reversed in VMAT2 deficient mutant mice PMID: 12716422
  19. physiological changes in VMAT2 levels or trafficking at the synapse may regulate dopamine release by altering the ratio of fillable-to-empty synaptic vesicles, as both continue to cycle in response to neural activity PMID: 15496457
  20. Our results demonstrate that genetic deletion of VMAT2 did not induce immediate cell death but did markedly inhibit dopamine transporter activity. PMID: 17664021
  21. these findings suggest that Vmat2 heterozygotes display a depressive-like phenotype that is devoid of anxiety-like behavior. PMID: 17898223
  22. demonstrates the efficacy of metabolomics as a functional genomics phenotyping tool for mouse models of neurological disorders, and indicates that mild reductions in the expression of VMAT2 affect normal brain metabolism PMID: 18041582
  23. female mice may have a greater amount/activity of VMAT2 function PMID: 18515015
  24. The ability of PACAP38 to increase VMAT2 expression suggests that PACAP38 signaling pathways may constitute a novel therapeutic approach to treat and prevent disorders of dopamine storage. PMID: 18533255
  25. Elevated markers of neurotoxicity in VMAT2 LO mice and cultures suggest that the capacity to store DA determines the amount of oxidative stress and neurodegeneration after METH administration. PMID: 18643795
  26. interaction between DAT and VMAT-2 function is more prevalent in female mice; and the +/- DAT mutation affects VMAT-2 function through an indirect mechanism, that does not involve an alteration in VMAT-2 protein or mRNA. PMID: 19615345

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Subcellular Location Cytoplasmic vesicle membrane; Multi-pass membrane protein.
Protein Families Major facilitator superfamily, Vesicular transporter family
Database Links

KEGG: mmu:214084

STRING: 10090.ENSMUSP00000026084

UniGene: Mm.268797

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