Recombinant Mouse Thimet oligopeptidase (Thop1)

Code CSB-YP807453MO
MSDS
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Source Yeast
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Code CSB-EP807453MO
MSDS
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Source E.coli
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Code CSB-EP807453MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP807453MO
MSDS
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Source Baculovirus
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Code CSB-MP807453MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Thop1; Thimet oligopeptidase; EC 3.4.24.15
Species
Mus musculus (Mouse)
Expression Region
1-687
Target Protein Sequence
MKPPAACAGD VVDAASPAST VNHLRWDLSA QQIRALTTQL IEQTKCVYDR VGAQNFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP CKDIRAASTE ADKKLSEFDV EMSMRQDVYQ RVVWLQEKTP KNSLKPEAAR YLERLIKLGR RNGLHLPQDT QEKIKNIKKR LSLLCIDFNK NLNEDTTFLP FTREELGGLP EDFLSSLEKA EDGKLKVTLK YPHYFPLLKK CHVPETRRLL EEAFNCRCKE ENCAILKELV SLRAQKSSLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ KLKPLGEQER AVILELKEAE CAKRGLPFDG RIHAWDMRYY MNQVEETRYR VDQNLLKEYF PMQVVTRGLL AIYQELLGLT FTLEEGAAAW HEDVRLYSVR DAASGEEIGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQLAVAAMVA NFTKPTPDAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF VEAPSQMLEN WVWEKEPLMR MSQHYRTGSE APQDLLEKLI KSRQANAGLF NLRQIVLAKV DQVLHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLSPKVGMD YRTSILRPGG SEDASAMLKQ FLGRDPKQDA FLLSKGLQVE GSEAPAC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the metabolism of neuropeptides under 20 amino acid residues long. Involved in cytoplasmic peptide degradation. Able to degrade the amyloid-beta precursor protein and generate amyloidogenic fragments.
Gene References into Functions
  1. the cellular peptidases dipeptidyl peptidase 3 (DPP-3) and thimet oligopeptidase 1 (TOP-1), both of which are present in nonimmunogenic necrotic cells, eliminated proteasomal degradation products and blocked Ag cross-presentation PMID: 24216478
  2. Data show that Thimet oligopeptidase (TOP) is coexpressed with estrogen receptor alpha, and estradiol regulates TOP expression in a brain region-specific manner in female mice. PMID: 20456597
  3. EP24.15 association with lipid rafts on the extracellular surface precedes constitutive release of the peptidase into the extracellular milieu for its action on neuropeptides PMID: 15287887
  4. Melanoma cells secrete thimet oligopeptidase which have an important role in tumor proliferation/angiogenesis in vitro and in vivo. PMID: 17620116
  5. increase in THOP1 expression might be part of a compensatory defense mechanism of the brain against an increased Abeta load. PMID: 18571100

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Subcellular Location
Cytoplasm.
Protein Families
Peptidase M3 family
Database Links
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