Recombinant Mouse Transcription factor 15 (Tcf15)

Code CSB-YP726672MO
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP726672MO
MSDS
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Source E.coli
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Code CSB-EP726672MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP726672MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP726672MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Tcf15
Uniprot No.
Alternative Names
Tcf15; Bhlhec2; Transcription factor 15; TCF-15; Meso1; Paraxis; Protein bHLH-EC2
Species
Mus musculus (Mouse)
Expression Region
1-195
Target Protein Sequence
MAFALLRPVG AHVLYPDVRL LSEDEENRSE SDASDQSFGC CEGLEAARRG PGPGSGRRAS NGAGPVVVVR QRQAANARER DRTQSVNTAF TALRTLIPTE PVDRKLSKIE TLRLASSYIA HLANVLLLGD AADDGQPCFR AAGGGKSAVP AADGRQPRSI CTFCLSNQRK GGSRRDLGGS CLKVRGVAPL RGPRR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Early transcription factor that plays a key role in somitogenesis, paraxial mesoderm development and regulation of stem cell pluripotency. Essential for the mesenchymal to epithelial transition associated with somite formation. Required for somite morphogenesis, thereby regulating patterning of the axial skeleton and skeletal muscles. Required for proper localization of somite epithelium markers during the mesenchymal to epithelial transition. Also plays a key role in regulation of stem cell pluripotency. Promotes pluripotency exit of embryonic stem cells (ESCs) by priming ESCs for differentiation. Acts as a key regulator of self-renewal of hematopoietic stem cells (HSCs) by mediating HSCs quiescence and long-term self-renewal. Together with MEOX2, regulates transcription in heart endothelial cells to regulate fatty acid transport across heart endothelial cells. Acts by forming a heterodimer with another helix-loop-helix (bHLH) protein, such as TCF3/E12, that binds DNA on E-box motifs (5'-CANNTG-3') and activates transcription of target genes.
Gene References into Functions
  1. Micrarray profiling on freshly isolated ECs had a genetic signature for microvascular heart ECs and identified Meox2/Tcf15 heterodimers as novel transcriptional determinants. This signature was similar in skeletal muscle and adipose tissue endothelium. PMID: 25561514
  2. The data demonstrate that PARAXIS initiates and stabilizes somite epithelialization by integrating signals from multiple pathways to control the reorganization of the ECM, cytoskeleton, and adhesion junctions during MET. PMID: 24038871
  3. Tcf15 contributes toward steering pluripotent cells in the direction of somatic lineages and that it will serve as a valuable tool for monitoring and interrogating early developmental transitions. PMID: 23395635
  4. may regulate early events during chondrogenesis by positively directing transcription of sclerotome-specific genes PMID: 15226298
  5. patterning events by basic helix-loop-helix-type transcription factors have deep impacts on regional chondrogenic and myogenic differentiation of somitic cells PMID: 17477400

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in heart and skeletal muscle. Specifically expressed in a subpopulation of embryonic stem cells (ESCs), that are still undifferentiated but primed for ifferentiation. Expressed in hematopoietic stem cells (HSCs).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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