Recombinant Mouse Transcription factor AP-2-beta (Tfap2b)

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Tfap2b

Uniprot No.

Q61313

Alternative Names

Tfap2b; Tcfap2bTranscription factor AP-2-beta; AP2-beta; Activating enhancer-binding protein 2-beta

Species

Mus musculus (Mouse)

Expression Region

1-459

Target Protein Sequence

MHSPPRDQAA IMLWKLVENV KYEDIYEDRH DGVPSHSSRL SQLGSVSQGP YSSAPPLSHT PSSDFQPPYF PPPYQPLPYH QSQDPYSHVN DPYSLNPLHQ PQQHPWGQRQ RQEVGSEAGS LLPQPRAALP QLSGLDPRRD YHSVRRPDVL LHSAHHGLDA GMGDSLSLHG LGHPGMEDVQ SVEDANNSGM NLLDQSVIKK VPVPPKSVTS LMMNKDGFLG GMSVNTGEVF CSVPGRLSLL SSTSKYKVTV GEVQRRLSPP ECLNASLLGG VLRRAKSKNG GRSLRERLEK IGLNLPAGRR KAANVTLLTS LVEGEAVHLA RDFGYICETE FPAKAVSEYL NRQHTDPSDL HSRKNMLLAT KQLCKEFTDL LAQDRTPIGN SRPSPILEPG IQSCLTHFSL ITHGFGAPAI CAALTALQNY LTEALKGMDK MFLNNTTNRH TSGEGPGSKT GDKEEKHRK

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-beta appears to be required for normal face and limb development and for proper terminal differentiation and function of renal tubular epithelia.

Gene References into Functions

AP-2beta is required in the periocular mesenchyme for normal development of the anterior segment of the eye. PMID: 27483349
AP-2 beta and beta-catenin interact both in vitro through GST pull-down assays and in vivo by co-immunoprecipitation. We further identified the interaction regions to the DNA-binding domain of AP-2 beta and the 1-9 Armadillo repeats of beta-catenin. PMID: 28277615
Tfap2b is associated with the development and remodeling of mouse ductus arteriosus and limb patterning. PMID: 21829553
PKD is thus a common modulator of the DNA binding activity of AP-2alpha and AP-2beta through their phosphorylation for negative regulation of the ABCA1 and adiponectin genes expression, respectively. PMID: 21345437
postprandial activation of PKCmicro plays a role in disordered postprandial adipocytokine expression through AP-2beta. PMID: 21424113
Data suggest that AP-2beta plays critical roles in the epinephrine phenotype and maturation of adrenal chromaffin cells. PMID: 20875861
Tfap2beta, Et-1, and Hif2alpha act in a transcriptional network during ductal smooth muscle development. Disruption of this pathway may contribute to patent ductus arteriosus by affecting development of smooth muscle in the ductus arteriosus. PMID: 18635823
AP-2beta transcriptional factor is a unique regulator of IRS-1 and a candidate gene for insulin resistance. PMID: 20097161
AP-2beta might modulate the expression of adiponectin by directly inhibiting its transcriptional activity PMID: 16954217
Hlxb9 and Tcfap2b were identified as genes whose expression was elevated in the ZPA compared to the rest of the developing limb bud. PMID: 17911046
AP-2beta is a candidate gene for producing adipocyte hypertrophy and may relate to the abnormal characteristics of adipocytes observed in obesity. PMID: 16373417

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Subcellular Location

Nucleus.

Protein Families

AP-2 family

Database Links

KEGG: mmu:21419

STRING: 10090.ENSMUSP00000027059

UniGene: Mm.137021

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Recombinant Mouse Transcription factor AP-2-beta (Tfap2b)

Product Details

Customer Reviews and Q&A

Target Background

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