Recombinant Mouse Transcription factor ETV6 (Etv6)

Code CSB-YP007860MO
MSDS
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Source Yeast
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Code CSB-EP007860MO
MSDS
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Source E.coli
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Code CSB-EP007860MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007860MO
MSDS
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Source Baculovirus
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Code CSB-MP007860MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Etv6; Tel; Tel1Transcription factor ETV6; ETS translocation variant 6; ETS-related protein Tel1; Tel
Species
Mus musculus (Mouse)
Expression Region
2-485
Target Protein Sequence
SETPAQSSI KQERISYTPP ESPVASHRSS TPLHVHTVPR ALRMEEDSIH LPTHLRLQPI YWSRDDVAQW LKWAENEFSL RPIESNKFEM NGKALLLLTK EDFRYRSPHS GDVLYELLQH ILKQRKSRML FSPFFPPGDS IHTKPEVLLH QNHDEDNCVQ RTPRTPAESV HHNPPTIELL HRPRSPITTN HRPSPDPEQQ RPQRSPLDNM SRRLSPVEKA QGPRLQQENN HQETYPLSVS PVENNHCLPS SPWQESTRVI QLMPSPIMHP LILNPRHSHS VDFKQSRHSE DGMNREGKPI NLSHREDLAY LNHIMVSMSP PEEHAMPIGR IADCRLLWDY VYQLLSDSRY ENFIRWEDKE SKIFRIVDPN GLARLWGNHK NRTNMTYEKM SRALRHYYKL NIIRKEPGQR LLFRFMKTPD EIMSGRTDRL EHLESQVLDE QTYQEDEPTI ASPVGWPRGN LPTGTAGGVM EAGELGVAVK EETRE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional repressor; binds to the DNA sequence 5'-CCGGAAGT-3'. Plays a role in hematopoiesis and malignant transformation.
Gene References into Functions
  1. Together with similar observations made in human PAX5-ETV6(+) B-ALLs, these data identified PAX5-ETV6 fusion as a potent oncoprotein that drives B-cell leukemia development. PMID: 28219927
  2. ETV6 DNA binding inhibition is established by the stability of its inhibitory helix PMID: 26920109
  3. ETV6-SAM domain interface mutations block ETV6-NTRK3-induced cellular transformation PMID: 23798677
  4. the autoinhibition of ETV6 DNA binding by sequences C-terminal to its ETS domain PMID: 22584210
  5. find that both IRS1 and kinase active IGF1R are required for ETV6-NTRK3 transformation, that tyrosine phosphorylated IRS1 is present in high molecular weight complexes with EN and IGF1R, and that EN colocalizes with IGF1R at the plasma membrane PMID: 21804605
  6. Cytogenetic study of 33 children with ETV6-RUNX1 positive acute lymphoblastic leukemia predict long term survival. PMID: 19998443
  7. TEL binding to IRF-8 for recruitment of HDAC3 is regulated by gamma-interferon PMID: 12370291
  8. role in accelerating differentiation of Friend erythroleukemia cells PMID: 12527908
  9. the ability of TEL to repress transcription and suppress growth is regulated by sumoylation and nuclear export PMID: 12626745
  10. nucleo-cytoplasmic delocalization of the major isoform of TEL-ETV6 is regulated by V-src PMID: 12893822
  11. ETV6-NTRK3.IRS-1 complex formation through the NTRK3 C terminus is essential for ETV6-NTRK3 transformation PMID: 14668342
  12. establish Tel/Etv6 as a selective and essential regulator of postembryonic hematopoietic stem cells PMID: 15371326
  13. leukemogenic effect of meningioma 1-TEL in our knock-in mice is pleiotropic, and the type of secondary mutation determines disease outcome PMID: 16105979
  14. Data suggest that TEL exerts a tumor suppressive function through augmenting the p53 pathway. PMID: 16828711
  15. alteration of the cellular localization of TEL may be a part of the cellular stress response and re-localization of TEL to the cytoplasm is an important step in the regulation of TEL PMID: 18022807
  16. Leukemia-related ETV6 expands erythroid precursors and stimulates hemoglobin synthesis. PMID: 19302286

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Subcellular Location
Nucleus.
Protein Families
ETS family
Database Links
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