Recombinant Mouse Negative regulator of reactive oxygen species (Nrros) is produced in an E.coli expression system. This partial protein covers the 29-651 amino acid region and comes with an N-terminal 6xHis tag, which makes purification and detection more straightforward. SDS-PAGE analysis shows the product reaches a purity level greater than 85%, suggesting it should be reliable for research applications. The product is intended for research use only. No endotoxin level has been specified.
Negative regulator of reactive oxygen species (Nrros) appears to play a crucial role in how cells manage oxidative stress by controlling reactive oxygen species production. This protein seems particularly significant for researchers studying cellular redox balance and oxidative stress pathways. Research on Nrros may provide insights into the mechanisms that protect cells from oxidative damage - something that's vital for understanding various physiological and pathological processes.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
Mouse LRRC33 (NRROS) is a transmembrane protein that requires precise folding, proper membrane integration, and specific tertiary structure for its functional activity in TGF-β activation and reactive oxygen species regulation. The E. coli expression system cannot provide the eukaryotic membrane environment or post-translational modifications required for this complex protein. The partial fragment (29-651aa) lacks the complete N-terminal region, including the signal peptide and transmembrane domain essential for proper membrane localization and function. The N-terminal 6xHis-tag may cause steric interference. The probability of correct folding with functional activity is extremely low.
1. Antibody Development and Validation
This application is highly suitable as antibody development relies on antigenic sequence recognition rather than functional protein folding. The large fragment (29-651aa) provides comprehensive epitope coverage for generating NRROS-specific antibodies.
2. Biochemical Characterization and Structural Studies
Basic biophysical analysis can be performed, but will not reflect the native NRROS structure. The lack of membrane context means results will describe an artificial soluble fragment rather than the membrane-associated protein.
Final Recommendation & Action Plan
This E. coli-expressed NRROS fragment is unsuitable for functional studies due to the essential requirements for membrane integration that cannot be met. The protein should not be used for interaction and functional studies. Application 2 provides only basic characterization of the fragment. Application 1 (antibody development) can proceed immediately. For reliable NRROS research, use a full-length protein expressed in mammalian systems with proper membrane integration.
