Product Details

Full Product Name

Rabbit anti-Mus musculus (Mouse) Nrros Polyclonal antibody

Uniprot No.

Q8BMT4

Target Names

Nrros

Alternative Names

Negative regulator of reactive oxygen species (Leucine-rich repeat-containing protein 33), Nrros, Lrrc33

Raised in

Rabbit

Species Reactivity

Mus musculus

Immunogen

Recombinant Mus musculus Nrros protein

Immunogen Species

Mus musculus (Mouse)

Conjugate

Non-conjugate

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (12-14 weeks)

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Key regulator of transforming growth factor beta-1 (TGFB1) specifically required for microglia function in the nervous system. Required for activation of latent TGF-beta-1 in macrophages and microglia: associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGFB1, and regulates integrin-dependent activation of TGF-beta-1. TGF-beta-1 activation mediated by LRRC33/NRROS is highly localized: there is little spreading of TGF-beta-1 activated from one microglial cell to neighboring microglia, suggesting the existence of localized and selective activation of TGF-beta-1 by LRRC33/NRROS. Indirectly plays a role in Toll-like receptor (TLR) signaling: ability to inhibit TLR-mediated NF-kappa-B activation and cytokine production is probably a consequence of its role in TGF-beta-1 signaling (Probable).

Gene References into Functions

NRROS-deficient (Nrros(-/-)) mice show defects in motor functions and die before 6 months of age. Nrros(-/-) mice display astrogliosis and lack normal CD11b(hi)CD45(lo) microglia, but they show no detectable demyelination or neuronal loss. perivascular macrophage-like myeloid cells populate the Nrros(-/-) CNS. Cx3cr1-driven deletion of Nrros shows its crucial role in microglial establishment during early embryonic stages. PMID: 28459434
this study presents NRROS as a novel negative regulator of RANKL-induced osteoclastogenesis. PMID: 26442864
results suggest that LRRC33 and TLRs may form a negative-feedback loop, which is important for the maintenance of immune homeostasis. PMID: 24550525
NRROS provides a hitherto undefined mechanism for regulating ROS production--one that enables phagocytes to produce higher amounts of ROS, if required to control invading pathogens, while minimizing unwanted collateral tissue damage PMID: 24739962

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Tissue Specificity

Mainly expressed in cells of hematopoietic origin, such as in immune organs such as lymph nodes, thymus and spleen. Among leukocytes, expressed at higher level in myeloid cell such as macrophages, neutrophils and dendritic cells. Highly expressed in centr

Database Links

KEGG: mmu:224109

STRING: 10090.ENSMUSP00000110817

UniGene: Mm.33498

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Nrros Antibody

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