Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Trib3

Uniprot No.

Q8K4K2

Research Area

Cardiovascular

Species

Mus musculus (Mouse)

Source

E.coli

Expression Region

1-354aa

Target Protein Sequence

MRATPLAASADVSCRKKPLEFDDNIDAKCPVLKRVRDEPEPGPLPSLLPPSPPPASDLSPAVAPATRLGPYILLEREQGSCSYRALHCPTGTEYTCKVYPASEAQAVLAPYARLPTHQHVARPTEVLLGSRLLYIFFTKTHGDLHSLVRSRRGIPESEAAGLFRQMASAVAHCHKHGLVLRDLKLRRFVFSNCERTKLVLENLEDACVMTGSDDSLWDKHACPAYVGPEILSSRPSYSGKAADVWSLGVALFTMLAGRYPFHDSEPVLLFGKIRRGTFALPEGLSAPARCLIRCLLRKEPSERLVALGILLHPWLREDHGRVSPPQSDRREMDQVVPDGPQLEEAEEGEVGLYG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

46.5 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The process of synthesizing the recombinant Mouse Trib3 protein includes transfecting e.coli cells with a DNA expression vector containing the gene for the desired protein (1-354aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is fused with a N-terminal 10xHis tag and C-terminal Myc tag. The recombinant Mouse Trib3 protein is then collected and purified from the cell lysate through affinity purification. It exhibits a purity exceeding 85%, as verified by SDS-PAGE.

Target Background

Function

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress. Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity. Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. Can inhibit APOBEC3A editing of nuclear DNA.

Gene References into Functions

Using SNRK transgenic and KO mouse models, study found that SNRK decreases cardiac metabolic substrate usage and mitochondrial coupling, while protecting against ischaemia/reperfusion injury. Effects on substrate usage and cell death are dependent on UCP3, which is downregulated through suppression of PPARalpha by Trib3, a novel binding partner of SNRK. PMID: 28117339
Overexpressed Trib3, specifically in skeletal muscle tissues, displayed impaired glucose homeostasis by suppressing insulin-stimulated glucose uptake. PMID: 30071535
under nutrient-limiting conditions that stimulate ATF4 activity, TRIB3 is implicated in the regulation of metabolic adaptation by restraining the transcription of Fgf21. PMID: 29378327
Data (including data from studies in transgenic and mutant mice) suggest that Akt2 plays critical role in type 1 diabetes in metallothionein-mediated preservation of cardiac insulin-stimulated metabolic signaling (insulin resistance) and preservation of cardiac function; this mechanism appear to involve inhibition of cardiac Trb3. (Akt2 = protein kinase Akt-2; Trb3 = tribbles pseudokinase 3) PMID: 29079702
restoration of TRB3 expression markedly abolished the effect of miR-124 on hepatic TG metabolism. PMID: 27845424
TRIB3, a single-domain protein with impaired kinase catalytic activity, has been described to perform a complex of roles, many of which are likely to be cell type-specific. (Review) PMID: 28393700
TRB3 regulates protein synthesis and breakdown via the Akt/mTOR/FoxO pathways in skeletal muscle. PMID: 28962861
Under physiological conditions, muscle TRIB3 also influences energy expenditure and substrate metabolism, indicating that the decrease and increase in muscle TRIB3 under fasting and nutrient excess, respectively, are critical for metabolic homeostasis. PMID: 27207527
In a knockout mouse model, Trib3 disruption modestly increased steady-state erythrocyte numbers and decreased mean corpuscular volume. PMID: 28062363
These data demonstrate that TRB3 inhibits insulin signaling in BAT, resulting in impaired differentiation and function. PMID: 26801556
TRB3 is a novel profibrotic mediator in SSc. TGF-beta induces TRB3, which in turn activates canonical TGF-beta/Smad signaling and stimulates the release of collagen, creating a positive feedback loop that may contribute to aberrant TGF-beta signalling in SSc. PMID: 25603829
study presents the finding that activation of the NF-kappaB pathway by Porphyromonas endodontalis LPS is important for Wnt5a expression in osteoblasts, and that TRIB3 may modulate the sustained expression of Wnt5a in osteoblasts stimulated by P. endodontalis PMID: 25601649
Trib3 interferes with the Parkinson's disease-associated prosurvival protein Parkin to mediate death PMID: 26224857
These observations support the notion that loss of TRIB3 is associated with a more aggressive phenotype in various types of tumors by enhancing the activity of the mTORC2/AKT/FOXO axis. PMID: 25168244
Tribbles 3 regulates the fibrosis cytokine TGF- beta 1 through ERK1/2-MAPK signaling pathway in diabetic nephropathy. PMID: 25133193
Fibroblasts from two long-lived mice mutants (Snell dwarf and PAPP-A knockout) expressed higher levels of TRB3 (a ATF4 target gene) during stress, suggesting a connection to longevity. PMID: 24691093
TRB3(-/-) islets show a decrease in both the amplitude and duration of cytokine-stimulated MLK3 induction and JNK activation. PMID: 25204656
Trib3 is upregulated in the mouse anterior piriform cortex in response to leucine-deficient diet. Deletion of Trib3 does not influence the rejection of amino acid-imbalanced diet. Trib3 is dispensable for spatial and reversal learning. PMID: 24732777
These findings suggest that TRB3 was involved in lipoapoptosis of INS-1 beta cell PMID: 24824999
show in renal tubular cells that TRB3 binds to mTOR and the rapamycin-insensitive companion of mTOR (Rictor), a protein specific to mTORC2 PMID: 24676635
TRB3 regulates muscle fiber type via a peroxisome proliferator-activated receptor-alpha (PPAR-alpha)-regulated miR499/miR208b pathway, revealing a novel function for TRB3 in the regulation of skeletal muscle fiber type and exercise capacity. PMID: 24740654
Data indicate that responding to Toll-like receptor 2 (TLR2)-specific stimulation, the expressions of cell death inducible putative kinase (NIPK) was significantly elevated. PMID: 25108431
TRIB3 mediated ER stress-induced beta-cell apoptosis via the NF-kappaB pathway. PMID: 24746137
observations presented here strongly support that TRIB3 plays a crucial role on THC anti-neoplastic activity PMID: 23567453
Trib3 mRNA expression in bone-marrow-derived mast cells is upregulated by IL-3. Degranulation & activation-induced cytokine mRNA expression are impaired in Trib3-deficient BMMCs. PMID: 23261831
TRB3 increased in diabetic testis and further increased in the testis of diabetic mice with Zn deficiency. PMID: 22000581
Silence of TRIB3 would diminish atherosclerotic burden and increase the plaque stability in diabetic mice. PMID: 22275087
emodin induces BV-2 cell apoptosis through TRB3 and consequently eliminates inflammatory microglia. PMID: 21275776
HHcy impairs hepatic glycogen synthesis by inducing the expression of TRB3 PMID: 21435438
the GCN2/eIF2alpha/ATF4 pathway is essential for the induction of the TRB3 gene transcription PMID: 21203563
enhanced reactive oxygen species and/or free fatty acids associated with the diabetic milieu induce podocyte C/EBP homologous protein and TRB3 expression PMID: 20660016
The pseudokinase tribbles homolog 3 interacts with ATF4 to negatively regulate insulin exocytosis in human and mouse beta cells. PMID: 20592469
MLK3 mediates its effects via the pseudokinase TRB3, a mammalian homolog of Drosophila Tribbles. PMID: 20421299
These data identify TRIB3 induction as a novel molecular mechanism in human insulin resistance and diabetes. PMID: 19996382
acute exercise reverses TRB3 expression and insulin signalling restoration in muscle PMID: 19681769
Knockdown of hepatic TRB-3 expression improved glucose tolerance, whereas hepatic overexpression of TRB-3 reversed the insulin-sensitive phenotype of PGC-1-deficient mice. PMID: 15107844
The combination of elevated TRB3 and constitutive hepatic p70 S6 kinase activity results in decreased insulin signaling via the insulin receptor substrate-1 protein/phosphatidylinositol 3-kinase/Akt pathway. PMID: 16887816
Results indicated that TRB3 plays a critical role in muscle differentiation. PMID: 17207467
Deletion of Trib3 has minimal effect on insulin-induced Akt activation in hepatic tissue. PMID: 17303803
Data show that TRB3 is an important negative regulator of adipogenesis that acts at an early step in the differentiation cascade to block the C/EBPbeta proadipogenic function. PMID: 17646392
TRB3 acts as a potent negative regulator of PPARgamma, a master regulator of adipocyte differentiation, and tightly controls adipogenesis PMID: 18187772
Data show that C/EBPbeta expression induced by insulin preceded that of TRB3 and that mutation of the C/EBPbeta binding site in TRB3 promoter abolished the responsiveness of the TRB3 gene to insulin. PMID: 19164449
TRB3 expression was reduced in the hepatic tissue from diet-induced obesity (Swiss) and leptin-deficient (ob/ob) mice, when compared with respective control groups at rest. PMID: 19492410
PPARalpha ligands may reduce cellular number by augmenting TRB3 expression, which in turn induces cell cycle arrest by reducing the expression of Cyclin B1. PMID: 19748123

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Subcellular Location

Nucleus.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily

Tissue Specificity

Highly expressed in liver. Not detected in heart, brain, spleen, lung, skeletal muscle, kidney or testis.

Database Links

KEGG: mmu:228775

STRING: 10090.ENSMUSP00000041747

UniGene: Mm.276018

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Code	CSB-EP818358MO
Abbreviation	Recombinant Mouse Trib3 protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Mouse Tribbles homolog 3 (Trib3)

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