Recombinant Mouse Ubiquitin carboxyl-terminal hydrolase DUB-1 (Dub1)

Code CSB-YP736802MO
MSDS
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Source Yeast
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Code CSB-EP736802MO
MSDS
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Source E.coli
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Code CSB-EP736802MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP736802MO
MSDS
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Source Baculovirus
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Code CSB-MP736802MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Usp17la
Uniprot No.
Alternative Names
Usp17la; Dub-1; Dub1; Ubiquitin carboxyl-terminal hydrolase 17-like protein A; USP17-A; EC 3.4.19.12; Deubiquitinating enzyme 1; Ubiquitin carboxyl-terminal hydrolase DUB-1; Ubiquitin thioesterase DUB-1; Ubiquitin-specific-processing protease DUB-1
Species
Mus musculus (Mouse)
Expression Region
1-526
Target Protein Sequence
MVVALSFPEA DPALSSPDAP ELHQDEAQVV EELTVNGKHS LSWESPQGPG CGLQNTGNSC YLNAALQCLT HTPPLADYML SQEHSQTCCS PEGCKLCAME ALVTQSLLHS HSGDVMKPSH ILTSAFHKHQ QEDAHEFLMF TLETMHESCL QVHRQSKPTS EDSSPIHDIF GGWWRSQIKC LLCQGTSDTY DRFLDIPLDI SSAQSVKQAL WDTEKSEELC GDNAYYCGKC RQKMPASKTL HVHIAPKVLM VVLNRFSAFT GNKLDRKVSY PEFLDLKPYL SEPTGGPLPY ALYAVLVHDG ATSHSGHYFC CVKAGHGKWY KMDDTKVTRC DVTSVLNENA YVLFYVQQAN LKQVSIDMPE GRINEVLDPE YQLKKSRRKK HKKKSPFTED LGEPCENRDK RAIKETSLGK GKVLQEVNHK KAGQKHGNTK LMPQKQNHQK AGQNLRNTEV ELDLPADAIV IHQPRSTANW GRDSPDKENQ PLHNADRLLT SQGPVNTWQL CRQEGRRRSK KGQNKNKQGQ RLLLVC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Deubiquitinating enzyme that removes conjugated ubiquitin from specific proteins to regulate different cellular processes. Has deubiquitinating enzyme activity for DNAH5, suggesting a role in the regulation of DNAH5 degradation by the ubiquitin-proteasome pathway. Has growth-suppressing activity; induces arrest in G1 phase upon controlled expression.
Gene References into Functions
  1. induced by cytokines in lymphocytes [review] PMID: 12447969
  2. DUB-1 contains a putative PEST motif and is polyubiquitinated, indicating that DUB-1 is also regulated by the ubiquitin-proteasome pathway. PMID: 18980247
Protein Families
Peptidase C19 family
Tissue Specificity
Expressed in hematopoietic progenitor cell lines Ba/F3 and FDCP1. Not detected in brain, lung, liver, kidney, thymus, spleen and bone marrow.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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