Recombinant Mouse Wee1-like protein kinase (Wee1)

Code CSB-YP026084MO
MSDS
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Source Yeast
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Code CSB-EP026084MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026084MO
MSDS
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Source Baculovirus
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Code CSB-MP026084MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wee1
Uniprot No.
Alternative Names
Wee1; Wee1-like protein kinase; EC 2.7.10.2; Wee1A kinase
Species
Mus musculus (Mouse)
Expression Region
1-646
Target Protein Sequence
MSFLSRQQPP PTRRVGAAYS LRQKLIFSPG SDCEEEEEEE EEGSGHSTGE DSAFQEPDSP LPSARSPAEA EAERRRRSPG AEPSSPGELE DDLLLQGGGG GAQAAGGGAE GDSWEEEGFG SSSPVKSPST AYFLSSPFSP VRCGGPGDAS PQGCGAPRAM DDPCSPQPDY PSTPPHKTFR KLRLFDTPHT PKSLLSKARV IDSGSVKLRG SSLFMDTEKS GKREFDTRQT PQVNINPFTP DPVLLHSSGR CRGRKRAYFN DSSEDMEASD YEFEDETRPA KRITITESNM KSRYTTEFHE LEKIGSGEFG SVFKCVKRLD GCIYAIKRSK KPLAGSVDEQ NALREVYAHA VLGQHPHVVR YFSAWAEDDH MLIQNEYCNG GSLADAISEN YRVMSYLTEV ELKDLLLQVG RGLRYIHSMS LVHMDIKPSN IFISRTSIPN AVSEEGDEDD WISNKVMFKI GDLGHVTRIS SPQVEEGDSR FLANEVLQEN YSHLPKADIF ALALTVVCAA GAEPLPRNGE QWHEIRQGRL PRIPQVLSQE VTELLRVMIH PDPERRPSAM ELVKHSVLLS ASRKSAEQLR IELNAEKFKN SLLQKELKKA QMAAKVAAEE RALFTDRMAT RSTTQSNRTS RLIGKKMNRS VSLTIY
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on 'Tyr-15'. Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase. Phosphorylation of cyclin B1-CDK1 occurs exclusively on 'Tyr-15' and phosphorylation of monomeric CDK1 does not occur. Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated. A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation.
Gene References into Functions
  1. Morphine significantly downregulated the expression of miRNA-219-5p, which targets WEE1 to suppress Tyr15 expressions and activate Cdc2, thus inhibiting the morphine-induced macrophage apoptosis. PMID: 27253431
  2. Results revealed that WEE1 is indispensable for maintaining genomic stability and it functions as a haploinsufficient tumor suppressor. PMID: 25088202
  3. Wee1 is directly or indirectly involved in the mechanism of the circadian rhythm-dependent changes in docetaxel-induced intestinal damage. PMID: 23892568
  4. The induction of Wee1 under hypoxia was confirmed both at the mRNA and protein levels. PMID: 21918363
  5. In this study, we found that the chromosomal wee1 gene is also down-regulated by KLF3. PMID: 22115574
  6. The regulation of Wee1 by SadA and SadB kinases is essential for the differentiation of polarized neurons. PMID: 20026642
  7. Mammalian Wee1 plays a critical role in maintaining genome integrity and is essential for embryonic survival at the pre-implantation stage of mouse development. PMID: 16810330
  8. expression of wee1 was directly regulated by the molecular components of the circadian clockwork in the regenerating liver PMID: 12934012
  9. transcription repressive activity of TBP-related factor 2 to wee1 promoter needs association with the promoter and TFIIA PMID: 17109819
  10. the suppression of nuclear import of cyclin B1, the induction of Wee1 kinase activity, and the transient nuclear accumulation of p21(CIP1/WAF1) may play important roles in the transient cell cycle delay in response to ionizing radiation PMID: 18425394

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Subcellular Location
Nucleus.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, WEE1 subfamily
Database Links
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