Recombinant Mouse Wilms tumor protein 1-interacting protein (Wtip)

Code CSB-YP752651MO
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP752651MO
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP752651MO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP752651MO
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP752651MO
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Wtip; Wilms tumor protein 1-interacting protein; WT1-interacting protein
Species
Mus musculus (Mouse)
Expression Region
1-398
Target Protein Sequence
MQRSRTAADD AALLLAGLGL RESEPTAGSP GRVRRGPRAV DEAAPASGRR GKGGCGGPEA APDVPSRPER GPRASLAGSD GGSARSSGIS LGYDQRHGPG PGPPSGGSAR SSVSSLGSRG SAGACADLLP PGVGPAPARS PEPAQFPFPL PSLPLPPGRE GGPSAAERRL EALTRELERA LEARTARDYF GICIKCGLGI YGARQACQAM GSLYHTDCFI CDSCGRRLRG KAFYNVGEKV YCQEDFLYSG FQQTADKCSV CGHLIMEMIL QALGKSYHPG CFRCSVCNEC LDGVPFTVDV DNNIYCVRDY HTVFAPKCAS CARPILPAQG CETTIRVVSM DRDYHVECYH CEDCGLQLSG EEGRRCYPLE GHLLCRRCHL RRLGQGPLPS PAVHVTEL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing. Negatively regulates Hippo signaling pathway and antagonizes phosphorylation of YAP1. Acts as a transcriptional corepressor for SNAI1 and SNAI2/SLUG-dependent repression of E-cadherin transcription. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. In podocytes, may play a role in the regulation of actin dynamics and/or foot process cytoarchitecture. In the course of podocyte injury, shuttles into the nucleus and acts as a transcription regulator that represses WT1-dependent transcription regulation, thereby translating changes in slit diaphragm structure into altered gene expression and a less differentiated phenotype. Involved in the organization of the basal body. Involved in cilia growth and positioning.
Gene References into Functions
  1. implicate Asxl1 in the maintenance of podocyte structure via its association with Wtip and in the regulation of WT1 signaling during early kidney development PMID: 26385183
  2. Dendrin is dispensable for the function of the normal glomerular filtration barrier, and it interacts with Wtip and Gadd45a. PMID: 24376653
  3. Wtip regulates the stable formation of cell adhesions to both extracellular matrix and neighboring cells and suggest that Wtip is necessary for normal glomerular filtration barrier function PMID: 21900451
  4. Podocyte injury induces nuclear translocation of WTIP via microtubule-dependent transport PMID: 20086015
  5. WTIP monitors slit diaphragm protein assembly as part of a multiple protein complex, linking this specialized adhesion junction to the actin cytoskeleton, and shuttles into the nucleus after podocyte injury PMID: 14736876
  6. Full-length Ror2 recruits Wtip to the cell membrane, a mutant involved in human disease fails to do so. PMID: 19785987

Show More

Hide All

Subcellular Location
Cell junction, focal adhesion. Cell junction, adherens junction. Nucleus. Cytoplasm, cytosol. Cell membrane.
Protein Families
Zyxin/ajuba family
Tissue Specificity
Expressed in kidney, lung, eye and ovary. In kidney, restricted to podocytes.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2025 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1