Product Details

Purity

>85% (SDS-PAGE)

Target Names

Xrcc6

Uniprot No.

P23475

Alternative Names

Xrcc6; G22p1; Ku70; X-ray repair cross-complementing protein 6; EC 3.6.4.-; EC 4.2.99.-; 5'-deoxyribose-5-phosphate lyase Ku70; 5'-dRP/AP lyase Ku70; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; Ku autoantigen protein p70 homolog; Ku70

Species

Mus musculus (Mouse)

Expression Region

2-608

Target Protein Sequence

SEWESYYKT EGEEEEEEEE SPDTGGEYKY SGRDSLIFLV DASRAMFESQ GEDELTPFDM SIQCIQSVYT SKIISSDRDL LAVVFYGTEK DKNSVNFKNI YVLQDLDNPG AKRVLELDQF KGQQGKKHFR DTVGHGSDYS LSEVLWVCAN LFSDVQLKMS HKRIMLFTNE DDPHGRDSAK ASRARTKASD LRDTGIFLDL MHLKKPGGFD VSVFYRDIIT TAEDEDLGVH FEESSKLEDL LRKVRAKETK KRVLSRLKFK LGEDVVLMVG IYNLVQKANK PFPVRLYRET NEPVKTKTRT FNVNTGSLLL PSDTKRSLTY GTRQIVLEKE ETEELKRFDE PGLILMGFKP TVMLKKQHYL RPSLFVYPEE SLVSGSSTLF SALLTKCVEK KVIAVCRYTP RKNVSPYFVA LVPQEEELDD QNIQVTPGGF QLVFLPYADD KRKVPFTEKV TANQEQIDKM KAIVQKLRFT YRSDSFENPV LQQHFRNLEA LALDMMESEQ VVDLTLPKVE AIKKRLGSLA DEFKELVYPP GYNPEGKVAK RKQDDEGSTS KKPKVELSEE ELKAHFRKGT LGKLTVPTLK DICKAHGLKS GPKKQELLDA LIRHLEKN

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA. Required for double-strand break repair and V(D)J recombination. Also has a role in chromosome translocation. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. During NHEJ, the XRCC5-XRRC6 dimer performs the recognition step: it recognizes and binds to the broken ends of the DNA and protects them from further resection. Binding to DNA may be mediated by XRCC6. The XRCC5-XRRC6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5-XRRC6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5-XRRC6 dimer together with APEX1 acts as a negative regulator of transcription. In association with NAA15, the XRCC5-XRRC6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

Gene References into Functions

Data show that phosphorylated Ku70 S155 interacts with the Aurora B kinase and leads to inhibition of its activity. PMID: 27849008
Ku70 is epistatic with XLF and DNA-PKcs and support a model in which inactivation of Ku70 allows DNA lesions to become accessible to alternative DNA repair pathways (other than Classical Non-Homologous End-Joining (C-NHEJ)). PMID: 28759779
Ku (ku70/ku80 heterodimer)roles in nonhomologous end joining and base excision repair pathways of DNA repair are overviewed. PMID: 25916111
A novel role for free Ku70 and free Ku80 in altering base excision repair. PMID: 24466051
A novel function of Ku70 essential for colon homeostasis. PMID: 23752193
Overexpression of Bcl2 rescues the hematopoietic stem cell defect in Ku70-deficient mice by restoration of quiescence. PMID: 24394664
important for DNA repair in somatic cells and in late spermatocytes of the testis PMID: 23857907
This study highlights Ku70 as an important player not only in maintaining genomic stability through NHEJ-dependent functions, but also in regulating pancreatic beta-cell proliferation, a novel NHEJ-independent function. PMID: 23474484
Ku70/80 binds to DNA double strand breaks (DSB) in all cell cycle stages and is likely actively displaced from DSB ends to free the DNA ends for DNA end resection and thus homologous recombination to occur. PMID: 22265216
The findings suggest that Artemis can work in not only the Ku-dependent repair process, but also the Ku-independent process at DNA double-strand break in living epithelial cells. PMID: 21820429
These findings clearly indicate that Ku70 plays a key role in regulation of the Ku80 expression level in and the radioresistance of lung epithelial cells. PMID: 21160137
Ku70/86 binds to the Apaf1 promoter and represses its activity. PMID: 20966962
a conserved critical Ku70 role for FOXO function toward coordination of a survival program PMID: 20570964
Ku70-deficient B cells have reduced, but still substantial, immunoglobulins class switch recombination PMID: 20142431
Ku can be identified as an effective 5'-dRP/AP lyase; data support an unexpected direct role for Ku in end-processing steps PMID: 20383123
Data show that DSB promote PP2A to associate with Ku 70 and Ku 86. PMID: 19794960
Antioxidants and lower oxygen tension reduced the high levels of neuronal death in primary cortical cultures derived from Ku70(-/-) mice, but not the low levels of cell death in wildtype cortical cultures PMID: 15342428
sites within the imprinting control center of the H19 and Igf2 genes bind Ku70/80 in a sequence-specific manner and with higher affinity than previously reported binding sites PMID: 15870260
The Ku70 was similarly decreased (by 2.1-, 1.5- and 1.7-fold, respectively) in Ku80+/- cells. PMID: 16014376
Ku70 has other antiapoptotic functions in Granzyme A (GzmA)-induced cell death, which are blocked when GzmA proteolyses Ku70 PMID: 16440001
Telomeres of Ku70(-/-) cells had a normal DNA structure and did not activate a DNA damage signal. PMID: 16845382
potential relevance of Bin1-Ku interaction to cancer are discussed in light of these findings PMID: 17671430
These observations suggest that the Ku heterodimer is important for longevity assurance in mice since divergent genetic backgrounds and/or environments likely account for these previously reported differences. PMID: 17875923
Defects in the NHEJ DNA repair pathway (Ku70 NHEJ-deficient gene) may participate in the disruption of cell cycle checkpoints leading to chromosomal instability and accelerated development of hepatocellular carcinoma. PMID: 18506893
Limited but statistically significant DNA rejoining was found in the liver and brain of Ku70-deficient mice 3.5 days after irradiation, showing the presence of a DNA double-strand break repair system other than non-homologous end joining. PMID: 18666816
These studies underscore an essential role of SIRT3 in the survival of cardiomyocytes in stress situations. PMID: 18710944
NRF-1 can also directly interact with poly(ADP-ribose) polymerase 1 (PARP-1) and co-purify the PARP-1.DNA-PK.Ku80.Ku70.topoisomerase IIbeta-containing protein complex. PMID: 19181665
Deleting Ku70 is milder than deleting Ku80 in p53-mutant mice and cells. PMID: 19330025

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Subcellular Location

Nucleus. Chromosome.

Protein Families

Ku70 family

Database Links

KEGG: mmu:14375

STRING: 10090.ENSMUSP00000068559

UniGene: Mm.288809

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Recombinant Mouse X-ray repair cross-complementing protein 6 (Xrcc6)

Product Details

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Target Background

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