Recombinant Mesomycoplasma hyopneumoniae 46 kDa surface antigen (p46)

The production of this Recombinant M.hyopneumoniae 232 mutT protein began at the genetic level, where the coding sequence for the mutT protein was first isolated and cloned into an expression plasmid vector. Recombinant DNA technology was used in the process. Next step was cloning. The expression vector must be introduced into the host cell (E.coli) so that the cells could be cultured and expressed the desired p46 protein. And we finally got the recombinant p46 protein with the purity of 90%+ determined by SDS-PAGE.

The crystal structure of P46, one of the main surface-antigen proteins, from M. hyopneumoniae is presented and shows N- and C-terminal α/β domains connected by a hinge. P46 has been identified as a novel natural killer cell–specific surface molecule that mediates cell activation. Beyond this, there are many findings that determined the various funcstions of P46 in cells. For example, it has been found that inactivation of runx3/p46 promotes cutaneous t-cell lymphoma; endomembrane targeting of human oas1 p46 augments antiviral activity; the cellular localization of the p42 and p46 oligoadenylate synthetase 1 isoforms and their impact on mitochondrial respiration; overexpression of the p46 (t1) translocase component of the glucose-6-phosphatase complex in hepatocytes impairs glycogen accumulation via hydrolysis of glucose 1-phosphate; enhanced expression of p46 shc in the nucleus and p52 shc in the cytoplasm of human gastric cancer.