Recombinant Myoblast determination protein 1 homolog (hlh-1)

Code CSB-YP326026CXY
MSDS
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Source Yeast
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Code CSB-EP326026CXY
MSDS
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Source E.coli
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Code CSB-EP326026CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326026CXY
MSDS
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Source Baculovirus
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Code CSB-MP326026CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
hlh-1
Uniprot No.
Alternative Names
hlh-1; B0304.1; Myoblast determination protein 1 homolog; MyoD protein 1; Helix-loop-helix protein 1
Species
Caenorhabditis elegans
Expression Region
1-324
Target Protein Sequence
MNTETSTQSA PSDTYDTSIY YNSSPRVTAN DITTLTSFAA PAPQVLDYAN TQYDIYRNQP AYYLPSYAPT APTTFYSDFA NFNVTRSQDF ASVPAVANSS DVKPIIIKQE KSTPNATELI IQSRVDSQHE DTTTSTAGGA GVGGPRRTKF VLSVDRRKAA TMRERRRLRK VNEAFEVVKQ RTCPNPNQRL PKVEILRSAI DYINNLERML QQAGKMTKIM EQNQHLQMTQ QINGAPPHDY VTSSHFASSS YNPENMFDDD DLTDSDDDRD HHKLGNAVDL RRRNSLDRLS RIVASIPNEE AMTDEQLLQP ANDVIDGEKK LEML
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in myogenesis, in cooperation with transcription factors unc-120 and hnd-1. Acts redundantly with fozi-1 to promote body wall muscle cell and coelomocyte specification in postembryonic mesoderm progenitors, probably through suppression of sem-2.
Gene References into Functions
  1. We found that HLH-1-dependent myogenic conversion specifically induced the expression of putative HLH-1-regulated chaperones in differentiating muscle cells. Moreover, disrupting the putative HLH-1-binding sites on ubiquitously expressed daf-21(Hsp90) and muscle-enriched hsp-12.2(sHsp) promoters abolished their myogenic-dependent expressio PMID: 28036392
  2. CeMyoD (HLH-1) is a myogenic regulatory factor that can convert almost all cells to a muscle-like fate, regardless of their lineage of origin. PMID: 15772130
  3. findings demonstrate that UNC-120/SRF and HND-1/HAND act in concert with HLH-1 to regulate myogenesis PMID: 17142668
  4. hlh-1 is indeed a direct target of PAL-1 in the posterior embryonic C. elegans muscle lineages, defining a novel in vivo binding site for this crucial developmental regulator PMID: 19261701
Subcellular Location
Nucleus.
Tissue Specificity
Body wall muscle cells; in clonal muscle precursors, in a set of early embryonic blastomeres (the ms-granddaughters), and in six glial-like cells called GLRS.
Database Links

KEGG: cel:CELE_B0304.1

STRING: 6239.B0304.1b

UniGene: Cel.6667

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