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Recombinant Neisseria meningitidis serogroup B Transferrin-binding protein 1 (tbp1), partial

Recombinant Neisseria meningitidis serogroup B Transferrin-binding protein 1 (tbp1), partial

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Code	CSB-EP881449NGG
Abbreviation	Recombinant Neisseria meningitidis serogroup B tbp1 protein, partial
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP881449NGG could indicate that this peptide derived from E.coli-expressed Neisseria meningitidis serogroup B (strain MC58) tbp1. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP881449NGG could indicate that this peptide derived from E.coli-expressed Neisseria meningitidis serogroup B (strain MC58) tbp1.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

tbp1

Uniprot No.

Q9K0U9

Research Area

Cell Biology

Alternative Names

tbp1; NMB0461; Transferrin-binding protein 1

Species

Neisseria meningitidis serogroup B (strain MC58)

Source

E.coli

Expression Region

22-300aa

Target Protein Sequence

AYAENVQAGQAQEKQLDTIQVKAKKQKTRRDNEVTGLGKLVKSSDTLSKEQVLNIRDLTRYDPGIAVVEQGRGASSGYSIRGMDKNRVSLTVDGVSQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSVEQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEALLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDSSNYAYFIVKEECKNGSYETCKANPKKDVVGKDERQTVSTRDYTGPNRF
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

37.0 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Neisseria meningitidis serogroup B (strain MC58) tbp1 protein expression in e.coli cells requires the insertion of a DNA fragment encoding the Neisseria meningitidis serogroup B (strain MC58) tbp1 protein (22-300aa) into a plasmid vector and the transferral of this vector into e.coli cells. The positive cells are screened, cultured, and then induced to express the tbp1 protein. The protein carries a N-terminal 10xHis tag and C-terminal Myc tag. The cells are lysed to harvest the recombinant Neisseria meningitidis serogroup B (strain MC58) tbp1 protein, which is purified through affinity purification and then detected by SDS-PAGE and subsequent staining of the gel with Coomassie Brilliant Blue. The purity of this recombinant Neisseria meningitidis serogroup B (strain MC58) tbp1 protein is over 85%.

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Target Background

Function

Neisseria acquires iron by extracting it from serum transferrin (TF) in its human host. Acts as a TF receptor and is required for TF utilization. Binds both apo- and holo-TF, via the TF C-terminus.

Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

TonB-dependent receptor family

Database Links

KEGG: nme:NMB0461

STRING: 122586.NMB0461

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