Recombinant Acinetobacter baumannii Beta-lactamase (NDM-1), partial

The DNA coding sequence translated into the Acinetobacter baumannii NDM-1 protein sequence (164-222aa) was fused with the N-terminal 6xHis-SUMO tag sequence to form the recombinant DNA, which was inserted into an expression vector. The reconstructed expression vector was transformed into the E.coli for follow-up expression. The product underwent purification to obtain the recombinant Acinetobacter baumannii NDM-1 protein with N-terminal 6xHis-SUMO tag. The SDS-PAGE analysis determined its purity higher than 90%. After electrophoresis, a 21 kDa protein band was observed on the gel.

NDM-1 is a gene providing instructions for making a protein Beta-lactamase in Acinetobacter baumannii. This gene is a novel metallo-beta-lactamase (MBL) gene carried by some Enterobacteriaceae that induces resistance to most of the antibiotics. This gene was first describled in a Swedish patient hospitalized in India with an infection due to Klebsiella pneumoniae. NDM-1 makes bacteria resistant to a broad range of beta-lactam antibiotics, including the antibiotics of the carbapenem family (a mainstay for the treatment of antibiotic-resistant bacterial infections).