Recombinant Oryza sativa subsp. japonica Curcuminoid synthase (Os07g0271500, LOC_Os07g17010)

Code CSB-YP847524OFG
MSDS
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Source Yeast
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Code CSB-EP847524OFG
MSDS
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Source E.coli
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Code CSB-EP847524OFG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP847524OFG
MSDS
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Source Baculovirus
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Code CSB-MP847524OFG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Os07g0271500
Uniprot No.
Alternative Names
Os07g0271500; LOC_Os07g17010; OJ1001_C01.122; OSJNBb0002J01.6Bisdemethoxycurcumin synthase; EC 2.3.1.211; Curcuminoid synthase
Species
Oryza sativa subsp. japonica (Rice)
Expression Region
1-402
Target Protein Sequence
MAPTTTMGSA LYPLGEMRRS QRADGLAAVL AIGTANPPNC VTQEEFPDFY FRVTNSDHLT ALKDKFKRIC QEMGVQRRYL HHTEEMLSAH PEFVDRDAPS LDARLDIAAD AVPELAAEAA KKAIAEWGRP AADITHLVVT TNSGAHVPGV DFRLVPLLGL RPSVRRTMLH LNGCFAGCAA LRLAKDLAEN SRGARVLVVA AELTLMYFTG PDEGCFRTLL VQGLFGDGAA AVIVGADADD VERPLFEIVS AAQTIIPESD HALNMRFTER RLDGVLGRQV PGLIGDNVER CLLDMFGPLL GGDGGGGWND LFWAVHPGSS TIMDQVDAAL GLEPGKLAAS RRVLSDYGNM SGATVIFALD ELRRQRKEAA AAGEWPELGV MMAFGPGMTV DAMLLHATSH VN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plant-specific type III polyketide synthase (PKS) that catalyzes the one-pot formation of the C6-C7-C6 diarylheptanoid scaffold of bisdemethoxycurcumin by the condensation of two molecules of 4-coumaroyl-CoA and one molecule of malonyl-CoA.
Gene References into Functions
  1. crystals of type III polyketide synthase belonged to space group P2(1), with unit-cell parameters a=72.7, b=97.2, c=126.2 A, alpha=gamma=90.0, beta=103.7 degrees PMID: 20693677
Protein Families
Chalcone/stilbene synthases family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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