Recombinant Oryza sativa subsp. japonica Gibberellin 20 oxidase 2 (20ox2)

Code CSB-YP612578OFG
MSDS
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Source Yeast
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Code CSB-EP612578OFG
MSDS
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Source E.coli
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Code CSB-EP612578OFG-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP612578OFG
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP612578OFG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
20ox2
Uniprot No.
Alternative Names
GA20OX2; 20ox2; C20ox2; GA20; Sd-1; SD1; Os01g0883800; LOC_Os01g66100; B1065E10.46Gibberellin 20 oxidase 2; OsGA20ox2; EC 1.14.11.-; GA 20-oxidase 2; Gibberellin C-20 oxidase 2; Os20ox2; Protein semidwarf-1
Species
Oryza sativa subsp. japonica (Rice)
Expression Region
1-389
Target Protein Sequence
MVAEHPTPPQ PHQPPPMDST AGSGIAAPAA AAVCDLRMEP KIPEPFVWPN GDARPASAAE LDMPVVDVGV LRDGDAEGLR RAAAQVAAAC ATHGFFQVSE HGVDAALARA ALDGASDFFR LPLAEKRRAR RVPGTVSGYT SAHADRFASK LPWKETLSFG FHDRAAAPVV ADYFSSTLGP DFAPMGRVYQ KYCEEMKELS LTIMELLELS LGVERGYYRE FFADSSSIMR CNYYPPCPEP ERTLGTGPHC DPTALTILLQ DDVGGLEVLV DGEWRPVSPV PGAMVINIGD TFMALSNGRY KSCLHRAVVN QRRERRSLAF FLCPREDRVV RPPPSAATPQ HYPDFTWADL MRFTQRHYRA DTRTLDAFTR WLAPPAADAA ATAQVEAAS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Key oxidase enzyme in the biosynthesis of gibberellin that catalyzes the conversion of GA53 to GA20 via a three-step oxidation at C-20 of the GA skeleton. Catalyzes the conversion of GA12 to GA9 via a three-step oxidation at C-20 of the GA skeleton. Contributes to the promotion of internode elongation in response to submergence via ethylene and gibberellin signalings.
Gene References into Functions
  1. a G to T single nucleotide polymorphism at position 38,382,746 in the sd1 locus on chromosome 1 of Koshihikari, causing a loss-of-function mutation of GA20-oxidase. PMID: 29850513
  2. Mutation of the SD1 gene is considered to be a key factor in controlling the protein content in rice grains. PMID: 25492221
Protein Families
Iron/ascorbate-dependent oxidoreductase family, GA20OX subfamily
Tissue Specificity
Strongly expressed in leaf blades, stems and unopened flowers. Lower expression in leaf sheath, rachis, shoot apex and roots.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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