Recombinant Oryza sativa subsp. japonica Squamosa promoter-binding-like protein 14 (SPL14)

Code CSB-YP762510OFG
MSDS
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Source Yeast
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Code CSB-EP762510OFG
MSDS
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Source E.coli
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Code CSB-EP762510OFG-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP762510OFG
MSDS
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Source Baculovirus
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Code CSB-MP762510OFG
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPL14
Uniprot No.
Alternative Names
SPL14; Os08g0509600; LOC_Os08g39890; B1168A08.33; OSJNBa0016N23.108; Squamosa promoter-binding-like protein 14
Species
Oryza sativa subsp. japonica (Rice)
Expression Region
1-417
Target Protein Sequence
MEMASGGGAA AAAGGGVGGS GGGGGGGDEH RQLHGLKFGK KIYFEDAAAA AGGGGTGSGS GSASAAPPSS SSKAAGGGRG GGGKNKGKGV AAAAPPPPPP PPRCQVEGCG ADLSGIKNYY CRHKVCFMHS KAPRVVVAGL EQRFCQQCSR FHLLPEFDQG KRSCRRRLAG HNERRRRPQT PLASRYGRLA ASVGEHRRFR SFTLDFSYPR VPSSVRNAWP AIQPGDRISG GIQWHRNVAP HGHSSAVAGY GANTYSGQGS SSSGPPVFAG PNLPPGGCLA GVGAATDSSC ALSLLSTQPW DTTTHSAAAS HNQAAAMSTT TSFDGNPVAP SAMAGSYMAP SPWTGSRGHE GGGRSVAHQL PHEVSLDEVH PGPSHHAHFS GELELALQGN GPAPAPRIDP GSGSTFDQTS NTMDWSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds to the SBP-box DNA core binding motif 5'-GTAC-3'. Can target the TCP motif 5'-TGGGCC/T-3' through interaction with PCF1 and PCF2. Key regulator of the plant architecture that controls shoot branching and panicle development. Promotes panicle branching. Promotes high grain yield. Binds to the promoters of TB1 and DEP1. Suppresses rice tillering mainly through positive regulation of TB1. Regulates plant height and panicle length through positive regulation of DEP1. Repressed by D53 in strigolactone (SL) signaling. Acts with D53 to mediate the SL-regulated tiller development. Functions as a direct downstream component of D53 in regulating tiller number and SL-induced gene expression. Binds directly to the D53 promoter and plays a critical role in the negative feedback regulation of SL-induced D53 expression. Involved in defense response against pathogens. Phosphorylated at Ser-163 in response to infection by the fungal pathogen Magnaporthe oryzae. Phosphorylation reduces SPL14/IPA1 binding to the GTAC site in the DEP1 promoter and enhances binding to the TGGGCC site in the WRKY45 promoter. Binding to the promoter of the pathogen defense gene WRKY45 activates its expression, leading to enhanced disease resistance. Reduces gibberellin-mediated disease susceptibility by stabilizing SLR1. Possesses transactivation activity in yeast cells.
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in young panicles. Expressed in the shoot apex at both the vegetative and reproductive stages. Highly expressed in the promordia of primary and secondary branches. Highly expressed in young panicles.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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