Recombinant Paralyzed arrest at two-fold protein 6 (pat-6)

Code CSB-YP522702CXY
MSDS
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Source Yeast
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Code CSB-EP522702CXY
MSDS
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Source E.coli
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Code CSB-EP522702CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522702CXY
MSDS
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Source Baculovirus
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Code CSB-MP522702CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pat-6
Uniprot No.
Alternative Names
pat-6; T21D12.4; Paralyzed arrest at two-fold protein 6; Actopaxin homolog; Parvin-like protein
Species
Caenorhabditis elegans
Expression Region
1-375
Target Protein Sequence
MSTLGRSKTP SRDEPKKPGV FEKLSGTLSR KKKAPEDEHG NQGGAHHATD EDEVLELELE GREALDQSLV PVLARNIWLE EGEIRRYLTK ETARDQKLAQ VVDLLIYWLN EELADQRIVV RHLQEDLFDG QIIQKLLEKL EQIRIEVPEV SQSEEGQRQK LQIVVQTANR ILGQPREQEK WSADLIHQKD FTAIIQLLVL LALHYRAPVR FPDNVVANVV VAQKEHGQVK THRITEQITT VQTELAPKGT RDAFDTLFDY GPDKLAHVKT SLLAFCNKHL NKINLEVSDL DNQFQDGVFL VLLVGLLEGY FVPLYHFNLQ VQSHEEKVKN VQFAFKLMED TGLEKPRSRV QDIANGDVKS TLRLLHLLFT KYKHI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the regulation of cell adhesion and cytoskeleton organization. Component of an integrin containing attachment complex, which is required for muscle development and maintenance. During embryonic development, required to recruit cpna-1, unc-89 and myofilaments to newly forming integrin attachments composed of integrins pat-2/pat-3, pat-4 and unc-112. Also required to reposition the integrin-based attachments so that they form the highly ordered array of dense body and M-line attachments that are characteristic of mature muscle cells. During the formation of neuromuscular junctions at the larval stage, negatively regulates membrane protrusion from body wall muscles.
Gene References into Functions
  1. PAT-6/actopaxin plays essential roles during the maturation of integrin-mediated muscle attachments in vivo. PMID: 12781130
Subcellular Location
Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, M line. Perikaryon. Cell projection, axon.
Protein Families
Parvin family
Tissue Specificity
Expressed from 1.5 stage embryos, mostly within the muscle cells. In adult hermaphrodites, expressed in the attachments of other muscles, including the uterine, anal depressor, anal sphincter, and vulval muscles, as well as in the spermatheca and the dist
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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