Recombinant Phosphate-binding protein pstS 3 (pstS3)

Code CSB-YP363779MVZ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP363779MVZ
MSDS
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Source E.coli
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Code CSB-EP363779MVZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP363779MVZ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP363779MVZ
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pstS3
Uniprot No.
Species
Mycobacterium tuberculosis
Expression Region
23-370
Target Protein Sequence
CGNDDNVT GGGATTGQAS AKVDCGGKKT LKASGSTAQA NAMTRFVNVF EQACPGQTLN YTANGSGAGI SEFNGNQTDF GGSDVPLSKD EAAAAQRRCG SPAWNLPVVF GPIAVTYNLN SVSSLNLDGP TLAKIFNGSI TQWNNPAIQA LNRDFTLPGE RIHVVFRSDE SGTTDNFQRY LQAASNGAWG KGAGKSFQGG VGEGARGNDG TSAAAKNTPG SITYNEWSFA QAQHLTMANI VTSAGGDPVA ITIDSVGQTI AGATISGVGN DLVLDTDSFY RPKRPGSYPI VLATYEIVCS KYPDSQVGTA VKAFLQSTIG AGQSGLGDNG YIPIPDEFKS RLSTAVNAIA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions in inorganic phosphate uptake, is probably the main carrier for phosphate uptake, it is the most highly expressed of the 3 PstS proteins under phosphate starvation. Binds phosphate; probably able to bind both H(2)PO(4)(-) and HPO(4)(2-). Part of the ABC transporter complex PstSACB involved in phosphate import. Probably plays a role in host phagosome maturation arrest.
Gene References into Functions
  1. Results suggest that PstS1-3 may play a role in the regulation of M. tuberculosis growth or metabolism while PstS3 and PknD contribute to the survival of the bacteria in phosphate poor conditions. PMID: 20933472
Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
PstS family
Database Links

KEGG: mtu:Rv0928

STRING: 83332.Rv0928

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