Recombinant Pongo abelii 2,3-cyclic-nucleotide 3-phosphodiesterase (CNP)

Recombinant Pongo abelii 2,3-cyclic-nucleotide 3-phosphodiesterase (CNP) is produced in E. coli, and the expression construct encodes the full-length mature Pongo abelii CNP protein comprising amino acids 1 to 418 and a 10xHis-tag at the N-terminus and a Myc-tag at the C-terminus. The two tags are helpful in recombinant CNP protein purification and detection. Through SDS-PAGE analysis, the purity of the recombinant Pongo abelii CNP protein is determined to be over 90%, appearing as a band with an approximate molecular weight of 55 kDa on the gel. This purified recombinant CNP protein is valuable for CNP-related neuroscience studies, enabling researchers to investigate the role and function of CNP in the context of neuronal processes.

CNP is primarily found in the myelin sheath of the central nervous system, where it participates in myelin formation and maintenance. It is essential for the proper development and function of oligodendrocytes, the cells responsible for producing myelin. CNP is also involved in promoting myelin compaction and stability and regulation of signaling pathways in the nervous system.