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Recombinant Propionibacterium acnes Chromosomal replication initiator protein DnaA (dnaA)

Recombinant Propionibacterium acnes Chromosomal replication initiator protein DnaA (dnaA)

Code	CSB-YP738033PSG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP738033PSG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP738033PSG-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP738033PSG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP738033PSG
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

dnaA

Uniprot No.

Q6ABL5

Alternative Names

dnaA; PPA0001Chromosomal replication initiator protein DnaA

Species

Propionibacterium acnes (strain KPA171202 / DSM 16379)

Expression Region

1-500

Target Protein Sequence

MSDTPFGDAD HPRPAPIHPD AVLPPPMSSQ SADNDPTEAL NEAWTNILTK VSKPNRAWLS NTTPVTMHSS TAMVAVPNEF ARDRLESKMR YELEELLSDH FHKAIHLAIT IDPDLELALG APDHEDEEEE VPPAQFVPKV TVGVTEPSAR PTTTIDDDEG NRLNPKYTFD SFVIGASNRF AHAAAVAVAE APGKSYNPLL IYGGSGLGKT HLLHAIGRYV MSYYDNVKVK YVSTEELTND FINAIGTNRT TEFRRSYRDV DVLLVDDIQF LQSKIQTQEE FFHTFNTLHN AQKQIVMTSD RPPKLLEALE PRLRSRFEWG LLTDIQPPDL ETRIAILRRK VAAEKITVEP DVLEFIASRI QTNIRELEGA LIRVTAFASL NQQPVDISLA EVVLKDLIPE GRETPVTPER IIAETADYFD ISADDLLGTS RAQTLVTARQ IAMYLCRELT DLSLPKIGAE FGGKDHTTVM HADRKIRALM GEQRQIFNQV SEITNRIKQY

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box): 5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phospholipids.

Subcellular Location

Cytoplasm.

Protein Families

DnaA family

Database Links

KEGG: pac:PPA0001

STRING: 267747.PPA0001

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