Recombinant Protease (pro)

Code CSB-YP344347SHN
MSDS
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Source Yeast
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Code CSB-EP344347SHN
MSDS
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Source E.coli
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Code CSB-EP344347SHN-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP344347SHN
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP344347SHN
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
pro
Uniprot No.
Alternative Names
pro; prt; Gag-Pro polyprotein [Cleaved into: Matrix protein p10; Phosphorylated protein pp24; Phosphorylated protein pp18; p12; Capsid protein p27; Nucleocapsid protein-dUTPase; NC-dUTPase; EC 3.6.1.23); Protease 17 kDa; EC 3.4.23.-); Protease 13 kDa; EC 3.4.23.-); G-patch peptide]
Species
Simian retrovirus SRV-2
Expression Region
1-314
Target Protein Sequence
SRKSLTTPSG KRDEGPAPGP EASLWGSQLC SSQQQQSISK LNRASPGSAG LDLCSTTHTV LTPEMGPQTL ATGVYGPLPP NTFGLILGRG STTVKGLQIY PGVIDNDYTG EFKIMARAIS SIITIPQGER IAQLVLLPLL RTAHKIQHPY RGDKNFGSSD IFWVQPITHQ KPSLVLWLDG KAFTGLIDTG ADVTIIKQED WPSHWPTTET LTNLRGIGQS NNPRQSSKYL TWKDKENNSG LIKPFVIPNL PVNLWGRDLL SQMKIMMCSP NDIVTAQMLA QGYSPGKGLG KREDGILQPI PNSGQLDRKG FGNF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Matrix protein.; Nucleocapsid protein p14: Nucleocapsid protein.; capsid protein.; The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.; The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.; Enhances the activity of the reverse transcriptase. May be part of the mature RT.
Subcellular Location
[Matrix protein p10]: Virion.; [Capsid protein p27]: Virion.; [Nucleocapsid protein-dUTPase]: Virion.; [Protease 13 kDa]: Virion.; [Protease 17 kDa]: Virion.
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