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Recombinant Protein kinase C-like 1B (pkc-1), partial

Recombinant Protein kinase C-like 1B (pkc-1), partial

Code	CSB-YP333948CXY
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP333948CXY
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP333948CXY-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP333948CXY
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP333948CXY
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

pkc-1

Uniprot No.

P34885

Alternative Names

pkc-1; kin-13; pkc-1b; ttx-4; F57F5.5Protein kinase C-like 1B; PKC1B; EC 2.7.11.13

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Involved in neuropeptide secretion in motor axons. Likely to act via the extracellular signal-regulated kinase/mitogen-activated protein kinase (ERK/MAPK) pathway in the signaling response to various sensory neurons; temperature, odor, taste, and osmolality. Its role in regulation differs depending on the neuron in which it is acting; thermosensation in AFD neurons, osmolality in ASH neurons, olfactory perception in AWA and AWC neurons. Promotes dauer formation mediated by the insulin/IGF pathway. Required for resistance to antimitotic toxins.

Gene References into Functions

Genetic analysis indicates that lin-45 and pkc-1 act together to regulate nose touch response; these results functionally link two conserved signaling pathways in adult C. elegans neurons and define distinct roles for PKC genes in vivo. PMID: 21143768

Subcellular Location

Membrane; Peripheral membrane protein. Cytoplasm, cytoskeleton. Note=Associated with membranes and the cytoskeleton.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Tissue Specificity

Expressed selectively in neurons that receive, transmit and process environmental signals.

Database Links

STRING: 6239.F57F5.5a.2

UniGene: Cel.19762

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