Recombinant Protein traI (traI)

Code CSB-YP309531ENL
MSDS
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Source Yeast
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Code CSB-EP309531ENL
MSDS
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Source E.coli
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Code CSB-EP309531ENL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP309531ENL
MSDS
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Source Baculovirus
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Code CSB-MP309531ENL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
traI
Uniprot No.
Alternative Names
traI; Protein TraI
Species
Escherichia coli
Expression Region
1-732
Target Protein Sequence
MIAKHVPMRS IKKSDFAELV KYITDEQGKT ERLGHVRVTN CEANTLPAVM AEVMATQHGN TRSEADKTYH LLVSFRAGEK PDAETLRAIE DRICAGLGFA EHQRVSAVHH DTDNLHIHIA INKIHPTRNT IHEPYRAYRA LADLCATLER DYGLERDNHE TRQRVSENRA NDMERHAGVE SLVGWIKREC LPELQAAQSW EDLHRVLREN GLKLRERGNG FIFEAGDGTT VKASTVSRDL SKPKLEARFG AFTPAEGGEA PRRREYRAKP LKTRIDTTEL YARYQSERQE MGAVRKGELD TLRRRRDRLI EAAMRSNRLR RAAIKLLGEG RIAKRLMYAQ AHKALRADLD KINREYRQGR QAVQERTQRR AWADWLKAEA MKGDDKALAA LRAREGRSDL KGNTIQGSGE AKPGHAAVTD NITKKGTIIY RVGSSAVRDD GDRLQVSREA TTDGLDAALR LAMERFGDRI TVNGTAEFKE RIAQAAAAGR LAITFDDAAL ERRRQELLTK EQAHEQPERN DGRRDRGGDG GIRPAAARTT LNATGGDGDR RDARAVSAGG TVALRKPNVG RIGRKPPPQS QNRLRALSQL GVVRIAGGAE MLLPRDVPGH VEQQGAEPAH ALRRGVSGPG RGLKPEQIAA AEKYVAEREQ KRLNGFDIPK HARYTDYVGA LSYAGTRNVE DQALALLRKE NDEILVLPVD KATVQRMKRL AIGDPVTVTP RGSLKTTRGR SR
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
The initiation process of transfer DNA synthesis requires the interaction of at least three plasmid-specific components (TraH, I, and J) at the transfer origin resulting in the assembly of a specialised nucleoprotein complex - the relaxosome. Site- and strand-specific cleavage at the transfer origin is dependent on TraI and TraJ.
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