Recombinant Protein unc-33 (unc-33), partial

Code CSB-YP312470CXY
MSDS
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Source Yeast
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Code CSB-EP312470CXY
MSDS
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Source E.coli
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Code CSB-EP312470CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP312470CXY
MSDS
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Source Baculovirus
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Code CSB-MP312470CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
unc-33
Uniprot No.
Alternative Names
unc-33; Y37E11C.1Protein unc-33; Inactive dihydropyrimidinase unc-33; Uncoordinated protein 33
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
During neurogenesis, plays an essential role in axonal guidance and outgrowth by regulating the polarization of both microtubule and actin cytoskeletons. Establishes the asymmetry of axonal and dendrite microtubules and the polarized sorting of neuronal proteins. This is achieved in part by regulating the localization of kinesin-like protein unc-104. In neurons without a distal microtubule-organizing center (MTOC), also controls the organization of microtubules in dendrites. During the dorso-ventral axonal guidance and outgrowth of VD neurons, required downstream of Rac GTPases ced-10 and mig-2 to inhibit growth cone filopodial protrusion mediated by the unc-6/netrin receptor unc-40-unc-5. Specifically, regulates growth cone filopodial protrusion polarity, and thus migration, by promoting F-actin polarization and by restricting plus-end microtubule accumulation in the growth cone. Probably downstream of mab-20/Sema2a and mab-20 receptor plx-2, regulates the guidance of DD/VD neuron axons by modulating fln-1 interaction with F-actin which results in the remodeling of the actin cytoskeleton. In hermaphrodites, involved in sex myoblast (SM) migration by regulating the gonad-dependent repulsion of SMs.; In neurons, required for the polarized sorting of axonal proteins. In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions. Plays a role in locomotion and egg-laying.; In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions.; In PLM neuron, regulates innexin unc-9 gap junction turnover by suppressing unc-9 transport out of gap junctions.
Gene References into Functions
  1. Here, using the C. elegans PLM neurons as a model, we show that UNC-44/ankyrin acts upstream of UNC-33/CRMP in regulation of a potential kinesin VAB-8 to control gap junction dynamics PMID: 27015090
  2. interacts with FLN-1 PMID: 25358863
  3. The results of this study suggest possible functions for LK as a regulator of neuritic elongation, corroborate roles for UNC-33/CRMP2 in the mechanism of LKE activity. PMID: 23825043
  4. We suggest that UNC-44 and UNC-33 direct polarized sorting through their global effects on neuronal microtubule organization. PMID: 22101643
  5. UNC-14/UNC-51 complex and Kinesin-1 are involved in the localization of UNC-33 in neurites PMID: 16236031

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Subcellular Location
Cell projection, axon. Cell projection, dendrite.; [Isoform a]: Cell projection, axon.
Protein Families
Metallo-dependent hydrolases superfamily, Hydantoinase/dihydropyrimidinase family
Tissue Specificity
Expressed in ventral cord and nerve ring (at protein level). Isoform a: Expressed in nerve ring (at protein level). Expressed in the nervous system, two amphid socket cells and weakly in non-neuronal pharyngeal cells.
Database Links
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