Recombinant Pseudomonas aeruginosa Beta-lactamase (ampC)

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Code CSB-EP326492EZX
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP326492EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) ampC.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP326492EZX could indicate that this peptide derived from E.coli-expressed Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) ampC.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
ampC
Uniprot No.
Research Area
Others
Alternative Names
ampC; PA4110Beta-lactamase; EC 3.5.2.6; Cephalosporinase
Species
Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
Source
E.coli
Expression Region
27-397aa
Target Protein Sequence
GEAPADRLKALVDAAVQPVMKANDIPGLAVAISLKGEPHYFSYGLASKEDGRRVTPETLFEIGSVSKTFTATLAGYALTQDKMRLDDRASQHWPALQGSRFDGISLLDLATYTAGGLPLQFPDSVQKDQAQIRDYYRQWQPTYAPGSQRLYSNPSIGLFGYLAARSLGQPFERLMEQQVFPALGLEQTHLDVPEAALAQYAQGYGKDDRPLRVGPGPLDAEGYGVKTSAADLLRFVDANLHPERLDRPWAQALDATHRGYYKVGDMTQGLGWEAYDWPISLKRLQAGNSTPMALQPHRIARLPAPQALEGQRLLNKTGSTNGFGAYVAFVPGRDLGLVILANRNYPNAERVKIAYAILSGLEQQGKVPLKR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
56.7kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Would you happen to know the activity of this beta lactamase against nitrocefin?

A:
Thanks for your inquiry. I'm afraid we haven't detect the activity of this beta lactamase against nitrocefin. Pls refer to: https://www.ncbi.nlm.nih.gov/pubmed/26142222

Target Background

Gene References into Functions
  1. Among several acquired beta-lactamase enzymes, the blaPER-1 and blaVEB-1, although produced less frequently, have clinical importance because of conferring resistance to oxyimino beta-lactams. PMID: 26944896
  2. Changes have been identified within AmpD responsible for derepression of ampC and conferring ceftazidime resistance. PMID: 27067331
  3. Pseudomonas aeruginosa is able to adapt to efficacious beta-lactams, including the newer cephalosporin ceftolozane, through a variety of mutations affecting its intrinsic beta-lactamase, AmpC. PMID: 26248364
  4. Overexpression of the ampC gene was observed in carbapenem-resistant P. aeruginosa isolates, but no carbapenem-susceptible isolates overexpressed the ampC gene. PMID: 22633564
Subcellular Location
Periplasm.
Protein Families
Class-C beta-lactamase family
Database Links

KEGG: pae:PA4110

STRING: 208964.PA4110

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