Code | CSB-EP319297EZX |
Abbreviation | Recombinant Pseudomonas aeruginosa eta protein, partial |
MSDS | |
Size | $388 |
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The process of synthesizing the recombinant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) eta protein includes transfecting e.coli cells with a DNA expression vector containing the gene for the desired protein (413-638aa). Subsequently, the cells are cultured to induce the expression of the intended protein. The protein is fused with a N-terminal 6xHis-KSI tag. The recombinant Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) eta protein is then collected and purified from the cell lysate through affinity purification. It exhibits a purity exceeding 85%, as verified by SDS-PAGE.
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KEGG: pae:PA1148
STRING: 208964.PA1148