Recombinant Pseudomonas putida Salicylate hydroxylase (nahG)

Code CSB-YP326049FFZ
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP326049FFZ-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP326049FFZ
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP326049FFZ
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nahG
Uniprot No.
Alternative Names
nahG; Salicylate hydroxylase; EC 1.14.13.1; Salicylate 1-monooxygenase
Species
Pseudomonas putida (Arthrobacter siderocapsulatus)
Expression Region
2-434
Target Protein Sequence
KNNKLGLRI GIVGGGISGV ALALELCRYS HIQVQLFEAA PAFGEVGAGV SFGPNAVRAI VGLGLGEAYL QVADRTSEPW EDVWFEWRRG SDASYLGATI APGVGQSSVH RADFIDALVT HLPEGIAQFG KRATQVEQQG GEVQVLFTDG TEYRCDLLIG ADGIKSALRS HVLEGQGLAP QVPRFSGTCA YRGMVDSLHL REAYRAHGID EHLVDVPQMY LGLDGHILTF PVRNGGIINV VAFISDRSEP KPTWPADAPW VREASQREML DAFAGWGDAA RALLECIPAP TLWALHDLAE LPGYVHGRVV LIGDAAHAML PHQGAGAGQG LEDAYFLARL LGDTQADAGN LAELLEAYDD LRRPRACRVQ QTSWETGELY ELRDPVVGAN EQLLGENLAT RFDWLWNHDL DTDLAEARAR LGWEHGGGGA LRQG
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

Can you please tell me the pricing and minimum order quantity for each of the products listed below? We do not need any tag. Are there any advantages/disadvantages to any of the 8 choices, such as stability? We need the protein to have good enzymatic activity with salicylate. Do you have any COA's from previous typical lots showing performance and specifications?

A:

1. Regarding the differences and advantages and disadvantages of several systems can refer to the web link: https://www.cusabio.com/c-20621.html
2. We have not tested the enzyme activity currently. We will try our best to express and purify in accordance with enzymatically active conditions, and try to obtain enzymatically active proteins. However, because the enzyme activity is not tested, there is still no guarantee that they must have enzyme activity.
3. Since the proteins are made-to-order, we cannot provide the COA of previous lot currently. The COA can be provided upon shipment.
4. It is recommended that you can order a small size first to have a try. The smallest size is 20ug.
5. Disclaimer about Tag Removal:
We can try to remove the tag by enzyme digestion, but we can't guarantee 100% success. The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal. If we succeed in removing the tag, we will charge for extra cost. If we fail in removing the tag, we won’t charge for any extra cost, and will only charge for the fusion protein cost.

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