Recombinant Rab-3-interacting molecule unc-10 (unc-10), partial

Code CSB-YP633799CXY
MSDS
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Source Yeast
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Code CSB-EP633799CXY
MSDS
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Source E.coli
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Code CSB-EP633799CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP633799CXY
MSDS
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Source Baculovirus
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Code CSB-MP633799CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
unc-10
Uniprot No.
Alternative Names
unc-10; T10A3.1; Rab-3-interacting molecule unc-10; Rim; Uncoordinated protein 10
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulates the efficiency of a post-docking step of the release pathway. Acts after vesicle docking likely via regulating priming. May regulate the conformational changes in syntaxin. Binding of vesicles via rab-3[GTP] to Rim may signal the presence of a docked synaptic vesicle. Rim may then signal to unc-13 to change the conformation of syntaxin from the closed to the open state. Syntaxin could then engage synaptobrevin on the docked vesicle to form SNARE complexes and to prime the vesicle for release. Not required for the development or the structural organization of synapses. May play a role in regulating entry into the dauer state.
Gene References into Functions
  1. we propose that the dense projection ensures proper coupling of primed vesicles with calcium signaling by retaining them at the active zone via unc-10 and SYD-2 dependent mechanisms PMID: 21430140
Subcellular Location
Cell junction, synapse.
Tissue Specificity
Restricted to discrete puncta in synapse-rich regions of the nervous system including the nerve ring, the ventral nerve cord and the dorsal nerve cord. Localized expression was found in the head.
Database Links

KEGG: cel:CELE_T10A3.1

STRING: 6239.T10A3.1a

UniGene: Cel.6180

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