Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

Mcpt2

Uniprot No.

P00770

Research Area

others

Alternative Names

Mcpt2; Mast cell protease 2; rMCP-2; EC 3.4.21.-; Group-specific protease; Mast cell protease II; rMCP-II

Species

Rattus norvegicus (Rat)

Source

E.coli

Expression Region

21-247aa

Target Protein Sequence

IIGGVESIPHSRPYMAHLDIVTEKGLRVICGGFLISRQFVLTAAHCKGREITVILGAHDVRKRESTQQKIKVEKQIIHESYNSVPNLHDIMLLKLEKKVELTPAVNVVPLPSPSDFIHPGAMCWAAGWGKTGVRDPTSYTLREVELRIMDEKACVDYRYYEYKFQVCVGSPTTLRAAFMGDSGGPLLCAGVAHGIVSYGHPDAKPPAIFTRVSTYVPWINAVINTSS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

30.5kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 21-247 form the expressed segment for recombinant Rat Mcpt2. The expected molecular weight for the Mcpt2 protein is calculated to be 30.5 kDa. Expression of this Mcpt2 protein is conducted in e.coli. The N-terminal 6xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of Mcpt2, which enables a simple process of detecting and purifying the Mcpt2 recombinant protein in the following steps.

Mast cell protease 2 (Mcpt2) is an enzyme belonging to the mast cell protease family, specifically the tryptase subgroup. In rats, Mcpt2 is primarily expressed in mast cells, which are immune cells involved in inflammatory and allergic responses. Mcpt2 functions as a serine protease and is stored in mast cell granules. Upon activation of mast cells, Mcpt2 is released into the surrounding tissues where it can contribute to various physiological processes, including inflammation, tissue remodeling, and defense against pathogens. Mcpt2 is implicated in allergic reactions and immune responses. Studying Mcpt2 and related mast cell proteases is essential for understanding the intricacies of mast cell function, immune regulation, and the development of therapeutic strategies for allergic and inflammatory disorders in which mast cells play a role.

Target Background

Function

This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.

Gene References into Functions

Phage display analysis showed that rMCP-2 is a chymase with a specificity similar to the corresponding enzyme in mice, mMCP-1, with a preference for Phe or Tyr in the P1 position, and a general preference for aliphatic amino acids both upstream and downstream of the cleavage site. PMID: 26114959
These results show both an effective and relatively rapid model of FA assessed by means of specific antibody titres and the high production of RMCP-II and its intestinal gene expression PMID: 25923134
Rapid expulsion of Trichinella spiralis first-stage larvae from rat intestine can occur in the absence of either intestinal mastocytosis or mast cell protease II release. PMID: 19812197

Protein Families

Peptidase S1 family, Granzyme subfamily

Database Links

KEGG: rno:29266

STRING: 10116.ENSRNOP00000027988

UniGene: Rn.9443

Code	CSB-EP360441RA
Abbreviation	Recombinant Rat Mcpt2 protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Rat Mast cell protease 2 (Mcpt2)

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