Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
Mcpt2; Mast cell protease 2; rMCP-2; EC 3.4.21.-; Group-specific protease; Mast cell protease II; rMCP-II
Species
Rattus norvegicus (Rat)
Expression Region
21-247aa
Target Protein Sequence
IIGGVESIPHSRPYMAHLDIVTEKGLRVICGGFLISRQFVLTAAHCKGREITVILGAHDVRKRESTQQKIKVEKQIIHESYNSVPNLHDIMLLKLEKKVELTPAVNVVPLPSPSDFIHPGAMCWAAGWGKTGVRDPTSYTLREVELRIMDEKACVDYRYYEYKFQVCVGSPTTLRAAFMGDSGGPLLCAGVAHGIVSYGHPDAKPPAIFTRVSTYVPWINAVINTSS
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 21-247 form the expressed segment for recombinant Rat Mcpt2. The expected molecular weight for the Mcpt2 protein is calculated to be 30.5 kDa. Expression of this Mcpt2 protein is conducted in e.coli. The N-terminal 6xHis tag and C-terminal Myc tag was smoothly integrated into the coding gene of Mcpt2, which enables a simple process of detecting and purifying the Mcpt2 recombinant protein in the following steps.
Mast cell protease 2 (Mcpt2) is an enzyme belonging to the mast cell protease family, specifically the tryptase subgroup. In rats, Mcpt2 is primarily expressed in mast cells, which are immune cells involved in inflammatory and allergic responses. Mcpt2 functions as a serine protease and is stored in mast cell granules. Upon activation of mast cells, Mcpt2 is released into the surrounding tissues where it can contribute to various physiological processes, including inflammation, tissue remodeling, and defense against pathogens. Mcpt2 is implicated in allergic reactions and immune responses. Studying Mcpt2 and related mast cell proteases is essential for understanding the intricacies of mast cell function, immune regulation, and the development of therapeutic strategies for allergic and inflammatory disorders in which mast cells play a role.
