Recombinant Rat Mast cell protease 2 (Mcpt2)

Code CSB-BP360441RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-EP360441RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-MP360441RA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mcpt2
Uniprot No.
Alternative Names
Mcpt2; Mast cell protease 2; rMCP-2; EC 3.4.21.-; Group-specific protease; Mast cell protease II; rMCP-II
Species
Rattus norvegicus (Rat)
Expression Region
21-247
Target Protein Sequence
IIGGVESIPH SRPYMAHLDI VTEKGLRVIC GGFLISRQFV LTAAHCKGRE ITVILGAHDV RKRESTQQKI KVEKQIIHES YNSVPNLHDI MLLKLEKKVE LTPAVNVVPL PSPSDFIHPG AMCWAAGWGK TGVRDPTSYT LREVELRIMD EKACVDYRYY EYKFQVCVGS PTTLRAAFMG DSGGPLLCAG VAHGIVSYGH PDAKPPAIFT RVSTYVPWIN AVINTSS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This enzyme, isolated from small intestine, specifically inactivates the apo forms of a certain group of intracellular pyridoxal phosphate-requiring enzymes. It has chymotrypsin-like specificity towards small substrates.
Gene References into Functions
  1. Phage display analysis showed that rMCP-2 is a chymase with a specificity similar to the corresponding enzyme in mice, mMCP-1, with a preference for Phe or Tyr in the P1 position, and a general preference for aliphatic amino acids both upstream and downstream of the cleavage site. PMID: 26114959
  2. These results show both an effective and relatively rapid model of FA assessed by means of specific antibody titres and the high production of RMCP-II and its intestinal gene expression PMID: 25923134
  3. Rapid expulsion of Trichinella spiralis first-stage larvae from rat intestine can occur in the absence of either intestinal mastocytosis or mast cell protease II release. PMID: 19812197
Protein Families
Peptidase S1 family, Granzyme subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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