Recombinant Rat 25-hydroxycholesterol 7-alpha-hydroxylase (Cyp7b1)

Code CSB-YP714349RA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP714349RA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP714349RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP714349RA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP714349RA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cyp7b1
Uniprot No.
Alternative Names
Cyp7b125-hydroxycholesterol 7-alpha-hydroxylase; EC 1.14.14.29; Cytochrome P450 7B1; Hippocampal transcript 1 protein; HCT-1; Oxysterol 7-alpha-hydroxylase; Fragment
Species
Rattus norvegicus (Rat)
Expression Region
1-414
Target Protein Sequence
ALEYQYVMKN PKQLSFEKFS RRLSAKAFSV KKLLTNDDLS NDIHRGYLLL QGKSLDGLLE TMIQEVKEIF ESRLLKLTDW NTARVFDFCS SLVFEITFTT IYGKILAANK KQIISELRDD FLKFDDHFPY LVSDIPIQLL RNAEFMQKKI IKCLTPEKVA QMQRRSEIVQ ERQEMLKKYY GHEEFEIGAH HLGLLWASLA NTIPAMFWAM YYLLQHPEAM EVLRDEIDSF LQSTGQKKGP GISVHFTREQ LDSLVCLESA ILEVLRLCSY SSIIREVQED MDFSSESRSY RLRKGDFVAV FPPMIHNDPE VFDAPKDFRF DRFVEDGKKK TTFFKGGKKL KSYIIPFGLG TSKCPGRYFA INEMKLLVII LLTYFDLEVI DTKPIGLNHS RMFLGIQHPD SDISFRYKAK SWRS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Oxysterol 7alpha-hydroxylase that mediates formation of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) from 25-hydroxycholesterol. Plays a key role in cell positioning and movement in lymphoid tissues: 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells.
Gene References into Functions
  1. estrogen suppresses CYP7B1-catalyzed 7alpha-hydroxylation of DEHA PMID: 25263657
  2. This study deminitrated that CYP7B1 mutations in patients with hereditary spastic paraplegia in French-Canadian. PMID: 22384504
  3. Abnormal expression of PPARalpha, CYP7B1 and ER may play a role in the pathogenesis of estrogen-induced intrahepatic cholestasis. PMID: 21029595
  4. CYP7B1 specific activity is highly regulated but does not seem to be rate limiting for bile acid synthesis. PMID: 12029625
  5. in the rat CYP7B1 demonstrates diurnal rhythm and is regulated by bile acids and cholesterol PMID: 12759897
  6. Kidney CYP24 activity, possibly regulated by factors such as PTH, acts in concert with kidney CYP27B1 to control serum 1,25D levels. PMID: 12914530
  7. Hepatic secretion of cholesterol and ABCG5/G8 expression are strongly stimulated in hypophysectomized rats during treatment with thyroid hormone. PMID: 18395092

Show More

Hide All

Subcellular Location
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Protein Families
Cytochrome P450 family
Tissue Specificity
Highly expressed in brain; also expressed in liver and kidney.
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1