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Recombinant Rat Aldose reductase-related protein 1 (Akr1b7)

Recombinant Rat Aldose reductase-related protein 1 (Akr1b7)

Code	CSB-YP732836RA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP732836RA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP732836RA-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP732836RA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP732836RA
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Akr1b7

Uniprot No.

Q5RJP0

Alternative Names

Akr1b7; Akr1b14Aldo-keto reductase family 1 member B7; EC 1.1.1.21; Aldehyde reductase; Aldose reductase-like protein AKR1B14; Aldose reductase-related protein 1

Species

Rattus norvegicus (Rat)

Expression Region

1-316

Target Protein Sequence

MTTFVKLRTK AKMPLVGLGT WKSPPGQVKE AVKAAIDAGY RHFDCAYVYQ NESEVGEAIQ EKIKEKAVRR EDLFIVSKLW STFFEKSLMK EAFQKTLSDL KLDYLDLYLI HWPQGLQAGK EFLPKDSQGK VLMSKSTFLD AWEGMEELVD QGLVKALGVS NFNHFQIERL LNKPGLKHKP VTNQVECHPY LTQEKLIQYC HSKGIAVIAY SPLGSPDRPY AKPEDPVVLE IPKIKEIAAK HKKTIAQVLI RFHVQRNVAV IPKSVTLSHI KENIQVFDFQ LSEEDMAAIL SLNRNWRACG LFVTSDEEDF PFHEEY

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Reduces a broad range of aliphatic and aromatic aldehydes to the corresponding alcohols. Reduces prostaglandins. May play a role in the metabolism of xenobiotic aromatic aldehydes.

Gene References into Functions

The bile acid binding to AKR1B14 mainly accelerates the NADP(+) dissociation, the rate-limited step of the enzyme reaction PMID: 21586312
crystal structure of the AKR1B14 His269Arg mutant in complex with NADPH was refined at 1.87 A degrees resolution PMID: 22505406
AKR1B14 was crystallized from polyethylene glycol solutions using the hanging-drop vapour-diffusion method and an X-ray diffraction data set was collected to 1.86 A resolution. PMID: 19342790
Aldo keto reductase 1B7 and prostaglandin F2alpha are regulators of adrenal endocrine functions PMID: 19809495
expression in rat vas deferens is barely detectable and is not androgen sensitive PMID: 12193556
expression of akr1b7 mRNA in rat liver is sexually differentiated, expressed in females but not in males, and regulated by the sexually dimorphic secretion pattern of GH. PMID: 15358674