Product Details

Purity

>85% (SDS-PAGE)

Target Names

Aifm1

Uniprot No.

Q9JM53

Alternative Names

Aifm1; Aif; Pdcd8; Apoptosis-inducing factor 1; mitochondrial; EC 1.6.99.-; Programmed cell death protein 8

Species

Rattus norvegicus (Rat)

Expression Region

102-612

Target Protein Sequence

GLSPEEKQR RAIASAAEGG SVPPIRVPSH VPFLLIGGGT AAFAAARSIR ARDPGARVLI VSEDPELPYM RPPLSKELWF SDDPNVTKTL QFRQWNGKER SIYFQPPSFY VSAQDLPHIE NGGVAVLTGK KVVHLDVRGN MVKLNDGSQI TFEKCLIATG GTPRSLSAID RAGAEVKSRT TLFRKIGDFR ALEKISREVK SITVIGGGFL GSELACALGR KSQASGIEVI QLFPEKGNMG KILPEYLSNW TMEKVKREGV KVMPNAIVQS VGVSGGKLLI KLKDGRKVET DHIVTAVGLE PNVELAKTGG LEIDSDFGGF RVNAELQARS NIWVAGDAAC FYDIKLGRRR VEHHDHAVVS GRLAGENMTG AAKPYWHQSM FWSDLGPDVG YEAIGLVDSS LPTVGVFAKA TAQDNPKSAT EQSGTGIRSE SETESEASEI TIPPSDPAVP QVPVEGEDYG KGVIFYLRDK VVVGIVLWNV FNRMPIARKI IKDGEQHEDL NEVAKLFNIH ED

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Binds to DNA in a sequence-independent manner. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates caspase-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. In contrast, participates in normal mitochondrial metabolism. Plays an important role in the regulation of respiratory chain biogenesis by interacting with CHCHD4 and controlling CHCHD4 mitochondrial import.

Gene References into Functions

Study provides evidence that RIP3 combined with AIF and translocated into the nucleus after cerebral ischemia/reperfusion (I/R) injury inducing ischemic neuronal DNA degradation and programmed necrosis. PMID: 27377128
Results suggest that apoptosis-inducing factor nuclear translocation and neuronal cell death are associated with Iduna loss after severe hypoxic-ischemic in the immature brain. PMID: 28108258
These data indicates that Drp1 mediates compression-induced programmed necrosis of NP cells by promoting mitochondrial translocation of p53 and nuclear translocation of AIF. PMID: 28411026
involved in streptomycin ototoxicity by translocation from mitochondria to cytosol and nucleus of cochlear hair cells PMID: 26963167
Data indicate that mitochondrial apoptosis-inducing factor (AIF) silencing prevents doxorubicin toxicity on cardiomyoblasts. PMID: 25283819
Peroxynitrite can trigger apoptosis in spiral ganglion neurons associated with mitochondrial AIF and endonuclease G intranuclear localization. PMID: 23006905
ERp57 forms complexes with mitochondrial mu-calpain, and ERp57-associated mitochondrial mu-calpain cleaves apoptosis-inducing factor to a truncated form. PMID: 18559257
In vivo inhibition of caspases and poly(ADP-ribose)polymerase-1, an upstream regulator of AIF release and calpain, proves the central role of the AIF-dependent pathway in lipopolysasccharide-induced nigral dopaminergic cell death. PMID: 20850531
The anti-apoptotic function of Hsp70 occurs through the inhibition of AIF-induced apoptosis by blocking the mitochondria to nucleus translocation of AIF PMID: 21387140
caspase inhibition inhibited the release of AIF; this suggests that there can be cross-talk between the caspase-dependent and the ostensibly caspase-independent pathway in neuronal apoptosis PMID: 19944742
A caspase-dependent, apoptotic pathway is important for non-hypertrophic cardiomyocytes (N-CMs) death, whereas a caspase-independent, AIF-mediated pathway plays a critical role in Hypertrophic cardiomyocyte (H-CM). PMID: 19633014
AIF translocation from mitochondria to the nucleus could be a key event leading to chromatin condensation and DNA degradation in cerebellar granule cells exposed to MeHg and H2O2. PMID: 12453066
AIF-mediated cell death may be an important mechanism of hypoxia-ischemia induced neuronal loss in the immature brain PMID: 12871572
Role of apoptosis-inducing factor in myocardial cell death in a model of ischemia-reperfusion in isolated hearts. PMID: 12963035
mitochondrial release of AIF may be an important factor, in addition to the previously reported cytochrome c and Smac, which could contribute to the selective vulnerability of CA1 neurons to transient global ischemic injury. PMID: 14526224
AIF translocated from mitochondria into the nucleus upon nitric oxide exposure. (apoptosis-inducing factor) PMID: 15379992
Zoledronic acid may be considered as an effective therapeutic agent in clinical trials of osteosarcoma, mitochondrial permeability in association with twranslocatino of apoptosis-inducing factor. PMID: 17050806
caspase-independent events are involved in cardiac apoptosis during the late remodeling stage of pathologic heart failure PMID: 17292393
May be a factor involved in skeletal muscle regeneration. PMID: 18508388
These results also suggested that JNK may be downstream of PARP activation and be required for PARP-mediated AIF. translocation. PMID: 18782186
An apoptosis-inducing factor (AIF)-related pathway is an essential target of PEDF-mediated neuroprotection. PMID: 18845835
Data show that restriction of dietary amino acids different from methionine decreases mitochondrial protein oxidative modification and AIF, and increases SIRT1, in rat liver. PMID: 19039676
The inhibition of Rho-kinase reduced cell apoptosis in I/R in vivo via suppression of JNK-mediated AIF translocation. PMID: 19061880
Data suggest that nuclear translocation of apoptosis inducing factor could underlie cell death, while fragmentation of caspase 3 could be associated with the morphological changes found in hypothalamic astrocytes of diabetic rats. PMID: 19094082
Cadmium-induced apoptosis is mediated by the translocation of AIF to the nucleus in rat testes. PMID: 19433269

Hide All

Subcellular Location

Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region.

Protein Families

FAD-dependent oxidoreductase family

Database Links

KEGG: rno:83533

STRING: 10116.ENSRNOP00000008503

UniGene: Rn.203165

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-YP872890RA
Size	Pls inquire
Source	Yeast
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP872890RA
Size	Pls inquire
Source	E.coli
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-EP872890RA-B
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-BP872890RA
Size	Pls inquire
Source	Baculovirus
Have Questions?	Leave a Message or Start an on-line Chat

Code	CSB-MP872890RA
Size	Pls inquire
Source	Mammalian cell
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rat Apoptosis-inducing factor 1, mitochondrial (Aifm1)

Product Details

Customer Reviews and Q&A

Target Background

×CloseMessage