Recombinant Rat BRCA1-A complex subunit RAP80 (Uimc1)

Code CSB-YP715939RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP715939RA
MSDS
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Source E.coli
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Code CSB-EP715939RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP715939RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP715939RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uimc1
Uniprot No.
Alternative Names
Uimc1; Rap80; BRCA1-A complex subunit RAP80; Receptor-associated protein 80; Ubiquitin interaction motif-containing protein 1
Species
Rattus norvegicus (Rat)
Expression Region
1-726
Target Protein Sequence
MPRRKKKIKE ASEGQNLEKK DLETTSSVSI KKKRRLEDLF IVISDSDGEE TKEENGLQKM KTKQSNRSKC LAKRKIAQMS EEEQFALALK MSEQEAKEVN NQEEKEEELL RKAIAESLNS RWSSDASAAR PRPLSTGPSS HPHQDSTKDS GTTEGIWQLA PPSLCKGSQI SQGNEAEEGK EPWDHSENPE EEPLSGSSGS QDQSSRPVFE NEKVKCFDRC TGHLAEHTQC GKPQESTGSG CAFPKAVQGR GDTSRQCLPT PADTKGLQDI GGTVHYYWGV PFCPAGVDPD QYTSVILCQL EVYQKSLKMA QRQLVKKRGF GEPVLPRPPF LIQNECGQEE QTSEKHEGLS EDVRAGAREE RQGSGASVWH SETKDSQKSP ITSLKQRLLL EEEPTTGRGQ SSQGLFVEET SEEVLKSSEG DDAVPASQSI AVLTSKRDLV LMPEASTEEV TVCPETQLSS FEPLDLNGED SVDGREIPTE LGMTVSNRQV DNREAGKDSR PPAVSASARV SCPLCNQDFP PTKIEQHAMY CTGLMEPETV LTRRRREAKK KSDGRTAAQP ALDISRKEKC YLCKSLVPLG EYQCHVETCL QLANVDRENG TEGMRRPRVC APVEGKPKQR LRKSKEKGHS QGRLLSLLEQ SEHRTTGVER TPKCSEAGAF RMPSPDVEEA GCSRERQSSL SKLNLNESPI KSFVPVSEAT NCLVDFKGQF AFRSQTKSGR GRRRKS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin-binding protein. Specifically recognizes and binds 'Lys-63'-linked ubiquitin. Plays a central role in the BRCA1-A complex by specifically binding 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. Also weakly binds monoubiquitin but with much less affinity than 'Lys-63'-linked ubiquitin. May interact with monoubiquitinated histones H2A and H2B; the relevance of such results is however unclear in vivo. Does not bind Lys-48'-linked ubiquitin. May indirectly act as a transcriptional repressor by inhibiting the interaction of NR6A1 with the corepressor NCOR1.
Subcellular Location
Nucleus.
Protein Families
RAP80 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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