Recombinant Rat Bile acid-CoA:amino acid N-acyltransferase (Baat)

Code CSB-YP720401RA
MSDS
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Source Yeast
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Code CSB-EP720401RA
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Source E.coli
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Code CSB-EP720401RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP720401RA
MSDS
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Source Baculovirus
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Code CSB-MP720401RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Baat
Uniprot No.
Alternative Names
BaatBile acid-CoA:amino acid N-acyltransferase; BACAT; BAT; EC 2.3.1.65; Bile acid-CoA thioesterase; Choloyl-CoA hydrolase; EC 3.1.2.27; Glycine N-choloyltransferase; Kan-1; Long-chain fatty-acyl-CoA hydrolase; EC 3.1.2.2
Species
Rattus norvegicus (Rat)
Expression Region
1-420
Target Protein Sequence
MAKLTAVPLS ALVDEPVHIR VTGLTPFQVV CLQASLKDDK GNLFNSQAFY RASEVGEVDL ERDSSLGGDY MGVHPMGLFW SMKPEKLLTR LVKRDVMNRP HKVHIKLCHP YFPVEGKVIS SSLDSLILER WYVAPGVTRI HVKEGRIRGA LFLPPGEGPF PGVIDLFGGA GGLFEFRASL LASHGFATLA LAYWGYDDLP SRLEKVDLEY FEEGVEFLLR HPKVLGPGVG ILSVCIGAEI GLSMAINLKQ ITATVLINGP NFVSSNPHVY RGKVFQPTPC SEEFVTTNAL GLVEFYRTFE ETADKDSKYC FPIEKAHGHF LFVVGEDDKN LNSKVHAKQA IAQLMKSGKK NWTLLSYPGA GHLIEPPYSP LCSASRMPFV IPSINWGGEV IPHAAAQEHS WKEIQKFLKQ HLNPGFNSQL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes the amidation of bile acids (BAs) with the amino acids taurine and glycine. More efficient at taurine conjugation of cholyl CoA than glycine conjugation. Amidation of BAs in the liver with glycine or taurine prior to their excretion into bile is an important biochemical event in bile acid metabolism. This conjugation (or amidation) plays several important biological roles in that it promotes the secretion of BAs and cholesterol into bile and increases the detergent properties of BAs in the intestine, which facilitates lipid and vitamin absorption. May also act as an acyl-CoA thioesterase that regulates intracellular levels of free fatty acids. In vitro, catalyzes the hydrolysis of long- and very long-chain saturated acyl-CoAs to the free fatty acid and coenzyme A (CoASH), and conjugates glycine to these acyl-CoAs.
Gene References into Functions
  1. is capable of forming both taurine and glycine bile acid conjugates and the enzyme is localized primarily in peroxisomes in rat liver PMID: 12951368
  2. hBAAT and rBaat are peroxisomal enzymes present in undetectable amounts in the cytosol. Unconjugated or deconjugated bile salts returning to the liver need to shuttle through the peroxisome before reentering the enterohepatic circulation. PMID: 17256745
Subcellular Location
Peroxisome. Cytoplasm, cytosol.
Protein Families
C/M/P thioester hydrolase family
Tissue Specificity
Expressed in liver (at protein level); found in hepatocytes, sinusoidal endothelial cells and Kupffer cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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