Recombinant Rat CD59 glycoprotein (Cd59)

Code CSB-YP004947RA
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Source Yeast
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Code CSB-EP004947RA
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Source E.coli
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Code CSB-EP004947RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004947RA
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Source Baculovirus
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Code CSB-MP004947RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cd59
Uniprot No.
Alternative Names
Cd59CD59 glycoprotein; MAC-inhibitory protein; MAC-IP; Membrane attack complex inhibition factor; MACIF; Protectin; CD antigen CD59
Species
Rattus norvegicus (Rat)
Expression Region
23-101
Target Protein Sequence
LRCYNCLD PVSSCKTNST CSPNLDACLV AVSGKQVYQQ CWRFSDCNAK FILSRLEIAN VQYRCCQADL CNKSFEDKPN N
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Potent inhibitor of the complement membrane attack complex (MAC) action. Acts at or after the C5b-8 stage of MAC assembly.
Gene References into Functions
  1. Study generated CD59(-/-) rats to investigate the role of CD59 in neuromyelitis optica spectrum disorders (NMO) and to create NMO pathology by passive transfer of AQP4-IgG. Results implicate an important role of CD59 in modulating NMO pathology in rats and demonstrate amplification of AQP4-IgG-induced NMO disease with CD59 knockout. PMID: 28212662
  2. Organ injury in neuromyelitis optica (NMO) seropositive CD59(-/-) rats was prevented by a complement inhibitor. Significant pathological changes in seropositive CD59(-/-) rats were not seen in optic nerve, spinal cord or brain, including circumventricular tissue. Results implicate a major protective role of CD59 outside of the central nervous system in seropositive NMO. PMID: 28750658
  3. Complement regulatory protein CD59 involves c-SRC related tyrosine phosphorylation of the creatine transporter in skeletal muscle during sepsis. PMID: 12219031
  4. generation of recombinant form and examination of efficient and specific inhibition of membrane attack complex activity in vivo PMID: 14519760
  5. In rats, CD59 and DAF genes are related to the negative effect of sulfasalazine on sperm maturation. PMID: 17166698

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Subcellular Location
Cell membrane; Lipid-anchor, GPI-anchor.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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