Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Activity

Not Test

Target Names

Cspg4

Uniprot No.

Q00657

Research Area

Others

Alternative Names

Chondroitin sulfate proteoglycan NG2;HSN tumor-specific antigen

Species

Rattus norvegicus (Rat)

Source

Yeast

Expression Region

575-1044aa

Target Protein Sequence

GPEIFQAYDPDSACEGLTIQLLGVSASVPVEHRDQPGEPVTEFSCRDLEAGNIVYVHRGGPAQDLTFRVSDGMQASGPATLKVVAVRPAIQILHNTGLRLAQGSAAAILPANLSVETNAVGQDVSVLFRVTGTLQFGELQKQGAGGVEGTEWWDTLAFHQRDVEQGRVRYLSTDPQHHTQDTVEDLTLEVQVGQETLSNLSFPVTIQRATVWMLQLEPLHTQNPHQETLTSAHLEASLEEEGEGGPYPHIFHYELVQAPRRGNLLLQGTRLSDGQSFSQSDLQAGRVTYRATTRTSEAAEDSFRFRVTSPPHFSPLYTFPIHIGGDPNAPVLTNVLLMVPEGGEGVLSADHLFVKSLNSASYLYEVMEQPHHGSLAWRDPKGRATPVTSFTNEDLLHGRLVYQHDDSETIEDDIPFVATRQGEGSGDMAWEEVRGVFRVAIQPVNDHAPVQTISRVFHVARGGQRLLTTD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

Protein Length

Partial

Tag Info

C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N-terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin-mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

Gene References into Functions

chondroitin sulfate proteoglycan 4 (CSPG4) is a potential mesenchymal progenitor cells marker. PMID: 27582000
These findings indicate that Col VI provided a characteristic microenvironment in the GOR and that NG2-Col VI interactions may regulate the differentiation of osteoblast lineages prior to terminal maturation. PMID: 27498042
studies suggest that proteoglycan-mediated entrapment upon NG2+ cells is an additional obstacle to CNS axon regeneration. PMID: 25471575
our data suggested that NG2 proteoglycan expression and secretion hallmarked demarcation as a process that actively separated necrosis from vital tissue and therefore decisively impacts secondary neurodegeneration after ischemic stroke. PMID: 22640018
In a transient 90-min middle cerebral artery occlusion (MCAO) model of rats, a large ischemic lesion is formed where macrophage-like cells massively accumulate, many of which express a macrophage marker, NG2 PMID: 19861972
results indicate that NG2 mediates the induction of iNOS and inflammatory cytokine expression, but not the chemokine expression in activated microglia. PMID: 19878709
NG2+ cells derived from the spinal cord following injury expressed high levels of laminin and fibronectin which promote neurite outgrowth. PMID: 20053907
identified a novel repeat named CSPG in the central ectodomain PMID: 12220645
Multiple protein domains of the NG2 proteoglycan inhibit neurite growth and induce growth cone collapse. PMID: 12514214
The expression patterns and cell associations of inhibitory CSPG4 have important implications for axon regeneration across acute and chronic spinal cord scar tissue. PMID: 12526031
NG2 is expressed by a distinct cell population in the mature central nervous system with the homogeneous antigenic phenotype of oligodendrocyte progenitors. PMID: 14572468
After peripheral nerve injury in rats and humans, an accumulation of NG2-positive cells was observed at the injury site. In the rat, there was an increase in NG2 glycanation for at least 2 weeks following injury. PMID: 14664826
Electroconvulsive seizures dramatically increased the proliferation of amygdala cells expressing the oligodendrocyte progenitor marker NG2. PMID: 15023573
PKC-alpha-mediated NG2 phosphorylation at Thr(2256) is a key step for initiating cell polarization and motility PMID: 15504744
Since NG2 loss in a rodent model of hypoxia-ischemia (H-I) occurs around the time of progressive cell death and appearance of the infarct, it may be that H-I rapidly induces a cellular response that actively depletes Ng2 from the hippocampal matrix. PMID: 15817274
NG2 is expressed by all perivascular cells along arterioles, and its absence denotes a venule-specific phenotype PMID: 15824037
Ectodomain shedding converts NG2 into a diffusible entity able to interact with the growth cone, and we suggest that this release is likely to enhance its axon growth-inhibitory activity PMID: 15866049
These data suggest that NG2+ MG in normal developing or pathologic brains are involved in the genesis or regeneration of the brain. PMID: 16534776
NG2 proteoglycan expression is transiently upregulated along venules during microvascular remodeling PMID: 16627368
These results suggest that NG2 positive cells which derived from subpial layer, may have some lineage to RG after SCI in adult rodents. PMID: 16902766
We propose that during development NG2(+)/A2B5(-) cells (pre-OPCs) represent the direct ancestor to A2B5(+) O2A progenitor cells (OPCs). PMID: 17503442
Differential phosphorylation of NG2 proteoglycan by ERK and Pkca helps balance cell proliferation and cell movement. PMID: 17591920
NG2 participates in the development and progression of glomerulosclerosis by stimulating proliferation of mesangial cells and deposition of extracellular matrix. PMID: 17686464

Hide All

Subcellular Location

Cell membrane; Single-pass type I membrane protein; Extracellular side. Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection, lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Cell surface.

Tissue Specificity

Neural cells and also extraneural tissues, especially in the developing mesenchyme.

Database Links

KEGG: rno:81651

STRING: 10116.ENSRNOP00000023326

UniGene: Rn.10381

Place an order now

I. Product details

Product name *

Code *

Size *

Quantity *

Message

II. Contact details

Name *

Email address *

Company/Organization

Phone

III. Ship To

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

IV. Bill To Same as Shipping Address

Address *

City *

State/Province *

Zip/Post code *

Country *

Attn Name *

Phone# *

CAPTCHA verification *

Code	CSB-YP006076RA
MSDS	MSDS
Size	$368
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Rat Chondroitin sulfate proteoglycan 4 (Cspg4), partial

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage