Recombinant Rat Complexin-1 (Cplx1)

Code CSB-YP005893RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005893RA
MSDS
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Source E.coli
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Code CSB-EP005893RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005893RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005893RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Cplx1
Uniprot No.
Alternative Names
Cplx1Complexin-1; Complexin I; CPX I; Synaphin-2
Species
Rattus norvegicus (Rat)
Expression Region
1-134
Target Protein Sequence
MEFVMKQALG GATKDMGKML GGDEEKDPDA AKKEEERQEA LRQAEEERKA KYAKMEAERE VMRQGIRDKY GIKKKEEREA EAQAAMEANS EGSLTRPKKA IPPGCGDEPE EEDESILDTV IKYLPGPLQD MFKK
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Positively regulates a late step in exocytosis of various cytoplasmic vesicles, such as synaptic vesicles and other secretory vesicles. Organizes the SNAREs into a cross-linked zigzag topology that, when interposed between the vesicle and plasma membranes, is incompatible with fusion, thereby preventing SNAREs from releasing neurotransmitters until an action potential arrives at the synapse. Also involved in glucose-induced secretion of insulin by pancreatic beta-cells. Essential for motor behavior.
Gene References into Functions
  1. actively drives SNARE complexes into linker-open conformation PMID: 30194295
  2. Results suggest that the C-terminal domain of Cplx1 plays a role in preferential membrane localization of Cpx to synaptic vesicles and find it as an important regulator for synaptic vesicle fusion through promoting Ca2+ in conjunction with SNAREs and synaptotagmins. PMID: 27821736
  3. Complexin-1 inhibits spontaneous release and synchronizes Ca2+-triggered synaptic vesicle fusion by distinct mechanisms. PMID: 25122624
  4. complexins modulate neuroexocytosis after assembly of membrane-bridging SNARE complexes PMID: 11751907
  5. CPX I plays a critical role in beta-cells in the control of the stimulated-exocytosis of insulin. PMID: 15126625
  6. Transient increases in the levels of complexin I and complexin II proteins were detected in the injured cerebral cortex 6 h following TBI. This increase was followed by a decrease of complexin I in the injured cortex and hippocampus. PMID: 16430375
  7. Results indicate that distinct functional domains of copmplexin I differentially regulate synaptic exocytosis, and that CplxI carries out a crucial role in fine-tuning Ca2+-triggered fast neurotransmitter release. PMID: 17828276
  8. Data show direct interaction of SNARE complex binding protein synaphin/complexin with calcium sensor synaptotagmin 1. PMID: 19132534

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Subcellular Location
Cytoplasm, cytosol. Cell junction, synapse, presynapse. Perikaryon.
Protein Families
Complexin/synaphin family
Tissue Specificity
Nervous system. Strongly expressed in brain, where it is predominant in neurons from cerebral cortex and thalamus (at protein level).
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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