Recombinant Rat DNA polymerase beta (Polb)

1. The I260Q variant of DNA polymerase beta is an efficient mutator polymerase with fairly indiscriminate misincorporation activities opposite all template bases. PMID: 23651085
2. The closed state of E295K has a more distorted active site than the active site in the wild-type Polb. PMID: 22651551
3. decline in pol beta activity started between 30 and 45 days postnatal in all the tissues. Post mitotic tissues showed pronounced decline than the proliferating tissues PMID: 22219134
4. at low pH the chemical step is rate limiting for catalysis, but at high pH, a postchemistry conformational step is rate limiting due to a pH-dependent increase in the rate of nucleotidyl transfer PMID: 22010960
5. These results point to the hinge region of DNA polymerase beta as being critical in the maintenance of the proper geometry of the dNTP binding pocket. PMID: 20108981
6. single stranded DNA interactions and kinetics PMID: 11700054
7. Changes in the activity and expression of DNA polymerase beta are mediated via cAMP and the A-kinase system, and that phosphorylation of this enzyme is also involved in this expression. PMID: 12088874
8. pol beta may have a role in UV lesion bypass and deregulated pol beta may enhance UV-induced genetic instability PMID: 12388548
9. data show that changes in liver expression of DNA polymerase beta, Ref-1, proliferating cell nuclear antigen, and Bax are associated with peroxisome proliferator-induced carcinogenesis in rats but not in hamsters. PMID: 12565796
10. pol beta binds to dsDNA oligomers with the site-size of the enzyme-dsDNA complex n = 5 +/- 1 base pairs PMID: 12741854
11. NMR data indicate that the nucleotide-DNA interaction appears to be essential for conformational activation PMID: 15248749
12. the 8-kDa domain had two inhibitor binding areas; Three amino acid residues (Lys60, Leu77, and Thr79) of the 8-kDa domain bound to LCA and compound 2 and four amino acid residues (Leu11, Lys35, His51, and Thr79) of the 8-kDa domain bound to compound 9. PMID: 15311928
13. Lys, Arg, Asp, Asn, and Glu substitutions for Ile at hinge residue 260 of pol beta yield enzymes with impaired activity or accuracy. PMID: 15751954
14. the subdomain-closing conformational change occurs before binding of the catalytic Mg(II) while the rate-limiting step occurs after binding of the catalytic Mg(II) PMID: 15794655
15. DNA polymerase beta discriminates the correct from incorrect substrate during the binding step. PMID: 15901725
16. The upregulation of base-excision repair activities after ischemic preconditioning was likely because of increased expression of DNA Polymerase beta. PMID: 16001017
17. data support a role for the flexible loop in pol beta error discrimination PMID: 16042415
18. describe a site-directed mutational analysis in which the key amino acids (L11, K35, H51, K60, L77, and T79), which are direct interaction sites in the domain, were substituted with K, A, A, A, K, and A, respectively PMID: 16996474
19. Loop II of pol beta functions to maintain accurate DNA synthesis by a direct or indirect influence on the nucleotide binding pocket. PMID: 19013261
20. for Pol beta, fidelity is dictated by the differences in free energy required to reach the highest energy transition state of the chemical step. PMID: 19231836
21. NMR assignments of the full-length protein (335 residues, 39 kDa) in the presence of a double gap--double hairpin DNA (22 nucleotides, 7 kDa) PMID: 19421423

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KEGG: rno:29240

STRING: 10116.ENSRNOP00000026039

UniGene: Rn.9346