Recombinant Rat Glutamate receptor, ionotropic kainate 5 (Grik5), partial

Code CSB-YP714121RA
MSDS
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Source Yeast
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Code CSB-EP714121RA
MSDS
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Source E.coli
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Code CSB-EP714121RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP714121RA
MSDS
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Source Baculovirus
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Code CSB-MP714121RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Grik5
Uniprot No.
Alternative Names
Grik5Glutamate receptor ionotropic; kainate 5; GluK5; Glutamate receptor KA-2; KA2
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Receptor for glutamate. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. The postsynaptic actions of Glu are mediated by a variety of receptors that are named according to their selective agonists. This receptor binds kainate > quisqualate > glutamate >> AMPA.
Gene References into Functions
  1. These findings indicate that ligand binding to only the GluK5 subunit is both necessary and sufficient to allow trafficking of recombinant GluK2/K5 heteromers to the cell membrane, but that occupancy of the GluK2 site alone is not. PMID: 23975096
  2. GluK2 and GluK5 assemble with 2:2 stoichiometry. PMID: 22509486
  3. CaMKII-dependent phosphorylation of GluK5 is responsible for synaptic depression by untrapping of KARs from the PSD and increased diffusion away from synaptic sites. PMID: 23288040
  4. Heteromeric GluK2/K5 receptors expressed in human HEK-293T cells show markedly higher glutamate sensitivity than GluK2 homomers and do not desensitize at low glutamate concentrations. PMID: 22114280
  5. kainic acid-preferring glutamate receptors are responsive to changes in plasma glucose concentrations and may participate in the activation of the paraventricular neurones during hypoglycaemic stress PMID: 15796762

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family, GRIK5 subfamily
Tissue Specificity
High expression in the cerebral cortex, pyriform cortex, caudate-putamen, hippocampal complex, medial habenulata and granule cell layer of the cerebellum. Weak expression in globus pallidus, thalamus, colliculi and the reticular thalamic nucleus.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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