Recombinant Rat Insulin-like growth factor-binding protein 1(Igfbp1)

In Stock
Code CSB-EP011095RAe1
Size US$1117Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names Igfbp1
Uniprot No. P21743
Research Area Signal Transduction
Alternative Names Igfbp1; Igfbp-1; Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1
Species Rattus norvegicus (Rat)
Source E.coli
Expression Region 26-272aa
Target Protein Sequence APQPWHCAPCTAERLELCPPVPASCPEISRPAGCGCCPTCALPLGAACGVATARCAQGLSCRALPGEPRPLHALTRGQGACVLEPAAPATSSLSGSQHEEAKAAVASEDELAESPEMTEEQLLDSFHLMAPSREDQPILWNAISTYSSMRAREITDLKKWKEPCQRELYKVLERLAAAQQKAGDEIYKFYLPNCNKNGFYHSKQCETSLDGEAGLCWCVYPWSGKKIPGSLETRGDPNCHQYFNVQN
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 26.9kDa
Protein Length Full Length of Mature Protein
Tag Info Tag-Free
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time 3-7 business days
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
(From Uniprot)
IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.
Gene References into Functions
  1. In simulated sleep apnea hypopnea syndrome, intermittent hypoxia in young rats does not cause physical growth retardation, but serum IGF-1 and IGFBP-3 levels decreased with the increase of hypoxia and decline of oxygen saturation. PMID: 22805023
  2. up-regulation of IGFBP-1 in oligodendrocytes in multiple sclerosis may serve two functions: (i) regulate IGF-1 actions, (ii) exert IGF-independent effects through its RGD sequence PMID: 20345750
  3. Insulin regulation of insulin-like growth factor-binding protein-1 gene expression PMID: 11784721
  4. regulation of expression by insulin is impaired by presence of hydrogen peroxide PMID: 11942857
  5. IGFBP-1 and IGFBP-3 are present in phosphorylated form in fasted rat skin, which induced inhibition of collagen biosynthesis in cultured fibroblasts PMID: 12670795
  6. IGFBP-1 released during the early steps of liver tissue damage and repair may interact with liver cells and potentiate the sensitivity of IGF-I to mitogenic signals. PMID: 15070850
  7. In this report we demonstrate that in H4IIE-C3 cells, four distinct classes of GSK-3 inhibitor mimic the effect of insulin on a third thymine-rich insulin response element-containing gene, IGFBP-1. PMID: 15350195
  8. Iron, radical oxygen species, and HIF-2 and -3 as well as prolyl hydroxylase pathways play important roles in mediating effects of hypoxia on IGFBP-1 gene expression in liver. PMID: 16166214
  9. insulin inhibition of IGFBP-1 mRNA levels can occur in the absence of the phosphorylation of Foxo1/Foxo3, whereas activation of the mTOR pathway is both necessary and sufficient PMID: 16455781
  10. Activation of the mTOR pathway, without activation of its upstream regulator PI 3-kinase, reduces IGFBP1 expression. PMID: 17032741
  11. Age-related oxidative stress underlies the upregulated JNK activation and IGFBP-1 expression. PMID: 17645865

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Subcellular Location Secreted.
Database Links

KEGG: rno:25685

STRING: 10116.ENSRNOP00000011338

UniGene: Rn.34026

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